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Global changes in gene expression in primary keratinocytes after KLF9 overexpression

ABSTRACT: To identify putative target genes of the transcription factor Klf9 in primary keratinocytes we over-expressed Klf9 in these cells using a lentiviral delivery system. We detected several hundred genes that show differential expression levels following ectopic Klf9 expression including several genes that are involved in proliferation and cell cycle control. These results allow insights into the mechanisms by which Klf9 regulates proliferation in primary keratinocytes. Primary human neonatal foreskin keratinocytes were infected with equal viral titers of a GFP and KLF9 expression construct, respectively. Cells were harvested 2,4 and 7 days after infection and total RNA was used to perform whole genome microarray analysis.

ORGANISM(S): Homo sapiens

SUBMITTER: Florian Spoerl 

PROVIDER: E-GEOD-36014 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Krüppel-like factor 9 is a circadian transcription factor in human epidermis that controls proliferation of keratinocytes.

Spörl Florian F   Korge Sandra S   Jürchott Karsten K   Wunderskirchner Minetta M   Schellenberg Katja K   Heins Sven S   Specht Aljona A   Stoll Claudia C   Klemz Roman R   Maier Bert B   Wenck Horst H   Schrader Annika A   Kunz Dieter D   Blatt Thomas T   Kramer Achim A  

Proceedings of the National Academy of Sciences of the United States of America 20120618 27

Circadian clocks govern a wide range of cellular and physiological functions in various organisms. Recent evidence suggests distinct functions of local clocks in peripheral mammalian tissues such as immune responses and cell cycle control. However, studying circadian action in peripheral tissues has been limited so far to mouse models, leaving the implication for human systems widely elusive. In particular, circadian rhythms in human skin, which is naturally exposed to strong daytime-dependent c  ...[more]

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