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ICLIP analysis of EZH2-interacting RNAs

ABSTRACT: RNAs directly interacting with EZH2 were isolated from human colorectal HCT116 cells using an in vivo crosslinking and immunoprecipitation strategy (iCLIP, König J et al, Nat Struct Mol Biol 2010) coupled to an ultrasequencing approach. RIP sequencing for 1 Human sample

ORGANISM(S): Homo sapiens

SUBMITTER: Antonio Gómez

PROVIDER: E-GEOD-36070 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Intronic RNAs mediate EZH2 regulation of epigenetic targets.

Guil Sònia S Soler Marta M Portela Anna A Carrère Jordi J Fonalleras Elena E Gómez Antonio A Villanueva Alberto A Esteller Manel M

Nature structural & molecular biology 20120603 7

Epigenetic deregulation at a number of genomic loci is one of the hallmarks of cancer. A role for some RNA molecules in guiding repressive polycomb complex PRC2 to specific chromatin regions has been proposed. Here we use an in vivo cross-linking method to detect and identify direct PRC2-RNA interactions in human cancer cells, revealing a number of intronic RNA sequences capable of binding to the core component EZH2 and regulating the transcriptional output of its genomic counterpart. Overexpres ...[more]

PMID: 22659877

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