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SmRNA under high osmotic stress

ABSTRACT: To identifiy osmotic stress responsive smRNAs, we used a deep-sequencing technique to profile small RNA populations in leaf and root tissues of plants under high osmotic stress and control conditions. We treated 30day-old plants with high osmotic stress and sampled leaves and roots from the same plant with 3 biologial replicates. Then, 3 replicates were pooled and total RNA was extracted and prepared for smRNA deep sequencing. After normalization and annotation, we selected potential osmotic stress responsive smRNAs.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Huan Wang

PROVIDER: E-GEOD-36560 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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IAA-Ala Resistant3, an evolutionarily conserved target of miR167, mediates Arabidopsis root architecture changes during high osmotic stress.

Kinoshita Natsuko N Wang Huan H Kasahara Hiroyuki H Liu Jun J Macpherson Cameron C Machida Yasunori Y Kamiya Yuji Y Hannah Matthew A MA Chua Nam-Hai NH

The Plant cell 20120907 9

The functions of microRNAs and their target mRNAs in Arabidopsis thaliana development have been widely documented; however, roles of stress-responsive microRNAs and their targets are not as well understood. Using small RNA deep sequencing and ATH1 microarrays to profile mRNAs, we identified IAA-Ala Resistant3 (IAR3) as a new target of miR167a. As expected, IAR3 mRNA was cleaved at the miR167a complementary site and under high osmotic stress miR167a levels decreased, whereas IAR3 mRNA levels incr ...[more]

PMID: 22960911

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