SmRNA under high osmotic stress
Ontology highlight
ABSTRACT:
ORGANISM(S): Arabidopsis thaliana
PROVIDER: GSE36560 | GEO | 2012/09/19
SECONDARY ACCESSION(S): PRJNA153439
REPOSITORIES: GEO
Similar Datasets
Project description:To identifiy osmotic stress responsive smRNAs, we used a deep-sequencing technique to profile small RNA populations in leaf and root tissues of plants under high osmotic stress and control conditions. We treated 30day-old plants with high osmotic stress and sampled leaves and roots from the same plant with 3 biologial replicates. Then, 3 replicates were pooled and total RNA was extracted and prepared for smRNA deep sequencing. After normalization and annotation, we selected potential osmotic stress responsive smRNAs.
2012-09-19 | E-GEOD-36560 | biostudies-arrayexpress
Project description:We performed a transcriptomic analysis on two tomato genotypes, M82 and Tondo, in response to a PEG-mediated osmotic treatment, mimicking water stress. The analysis was conducted separately on leaves and roots to characterize the specific response of these two organs. A total of 6,267 differentially expressed transcripts related to osmotic stress response was detected. The construction of gene co-expression networks defined the metabolic and signaling pathways of the common and specific responses of leaf and root. The common response was characterized by ABA-dependent and ABA-independent signaling pathways, and by the interconnection between ABA and JA signaling. The root specific response concerned genes involved in cell wall metabolism and remodeling, whereas the leaf specific response was principally related to leaf senescence and ethylene signaling. The transcription factors representing the hubs of these regulatory networks were identified. Some of them have not yet characterized and can represent novel candidates for tolerance. Finally, several genes showing a genotype-specific expression regulation in response to the treatment were detected. These genes may be involved in the different sensitivity to the osmotic treatment of the two tomato genotypes. In conclusion, this work shed new light on the regulatory networks occurring in tomato leaf and root under osmotic stress and set the base for an in-depth characterization of novel stress-related genes, that may represent potential candidates for improving tolerance to water stress in tomato.
2023-05-15 | GSE224629 | GEO
Project description:ced1 mutant has reduced expression of NCED3 in response to osmotic stress (polyethylene glycol) treatments compared to the wild type. Other ABA biosynthesis genes are also greatly reduced in ced1 under osmotic stress. We used microarrays to detail the global programme of gene expression under osmotic stress treatment and identified distinct classes of up/down-regulated genes in the stress pathway.
2010-12-08 | GSE25889 | GEO
Project description:Purpose:to identify the response of Frankia sp.strain CcI6 to salt and osmotic stress. Frankia sp.strain CcI6 was exposed to salt and osmotic stress for seven days. RNAseq analysis was carried out to ge an insight into the response of the bacterium under salt and osmotic stress conditons
2017-09-22 | GSE95217 | GEO
Project description:ced1 mutant has reduced expression of NCED3 in response to osmotic stress (polyethylene glycol) treatments compared to the wild type. Other ABA biosynthesis genes are also greatly reduced in ced1 under osmotic stress. We used microarrays to detail the global programme of gene expression under osmotic stress treatment and identified distinct classes of up/down-regulated genes in the stress pathway. Arabidopsis seedlings were selected at 16 days with 6hr PEG treatment for RNA extraction and hybridization on Affymetrix microarrays. From Norther data we have known some of well-known stress responsive gene were significantly changed. We sought to obtain whole gene expression to analysis which genes were affected in the osmotic stress pathway.
2010-12-08 | E-GEOD-25889 | biostudies-arrayexpress
Project description:Osmotic stress is detrimental for the plant which survival relies heavily on proteomic plasticity. Protein ubiquitination is a central post-translational modification (PTM) in osmotic mediated stress. Plants use the ubiquitin proteasome system to modulate protein contents required to respond to and tolerate adverse growth conditions and a role for ubiquitin to mediate endocytosis and trafficking of plant plasma membrane proteins has recently emerged. In this study, using the K-Ɛ-GG antibody enrichment method integrated with high-resolution mass spectrometry, a list of 786 ubiquitinated lysine (K-Ub) residues in 451 proteins of which 50 % were transmembrane proteins was compiled for Arabidopsis root membrane proteins, increasing the database of ubiquitinated substrates in plants. Such analysis allowed to evidence specific ubiquitination motifs, a role for ubiquitination in the internalization and sorting of cargo proteins and ubiquitination dialog with other post-translational modifications (PTMs). In silico interactomics analysis allowed to pinpoint two E2 ligases, UBC32 and UBC34 targeting membrane proteins and putatively involved in response to osmotic stress. The simultaneous quantification of proteome and ubiquitinome after plant treatment with mannitol, suggested a minor role for ubiquitination in protein degradation. Then, this study revealed that a major plasma membrane aquaporin (PIP2;1) harbors two ubiquitination sites that dialog with N-terminal acetylation and C-terminal phosphorylation, to putatively limit PIP2;1 degradation and to favor its internalization contributing to a decreased root hydraulic conductivity (Lpr) while maintaining its cellular abundance.
2022-04-04 | PXD022249 | Pride
Project description:The shoots and roots of a plant respond differently to osmotic stress, as they have distinct functions and anatomical structures. Under conditions of high solute concentration, such as in saline soils or drought, water uptake by the roots is reduced, resulting in cellular dehydration. In this study, we performed transcriptional profiling of roots of Arabidopsis under osmotic stress conditions such as high salinity and drought using mRNA-Seq for the assessment of gene expression changes in roots of Arabidopsis. mRNA-Seq analysis showed that many differentially expressed genes showed differential expressions under both salt stress and drought stress conditions in roots and were distinct from aerial parts. We confirmed 68 transcription factor genes which is involved in osmotic stress signal transduction in roots and are connected tightly. Interestingly, well-known ABA-dependent and/or -independent osmotic stress-responsive genes were less increased in roots, indicating that osmotic stress response in roots might be regulated by stress pathways other than well-known pathways. We identified 26 osmotic stress-responsive genes, which have alternative splicing variant isoforms, showed distinct expression in roots under osmotic stress conditions from the mRNA-Seq analysis. Quantitative RT-PCR confirmed that alternative splicing variants, such as ANNAT4, MAGL6, TRM19, and CAD9, have differential expressions in roots under osmotic stress conditions, indicating that alternative splicing is an important regulatory mechanism in osmotic stress response in roots. Taken together, our study suggest that many transcription factor families are involved in osmotic stress response in roots and tightly connected each other. In addition, alternative splicing and function of alternative splicing variant isoforms are also important in osmotic stress response in roots. To understand the alternative splicing mechanism in roots, further study is necessary.
2023-12-08 | GSE229217 | GEO
Project description:ced2 mutant has reduced expression of NCED3 in response to osmotic stress (PEG, polyethylene glycol) treatments compared to the wild type. Other ABA biosynthesis genes are also greatly reduced in ced2 under osmotic stress. We used microarrays to detail the global programme of gene expression under osmotic stress treatment and identified distinct classes of up/down-regulated genes in the stress pathway.
2013-02-21 | GSE44419 | GEO
Project description:The CAMTA1 mutant and Col-0 were studied under water and drought condition. The camta1 showed stunted primary root growth under osmotic stress. The expression analysis revealed drought recovery as major indicative pathway along with membrane and chloroplast related protein in camta1 under drought stress. Large number of positively regulated genes were related to osmotic balance, transporters, AP2 and ABA. We used Affymetrix expression analysis to validate the role of CAMTA1 under drought stress.
2012-08-13 | GSE40061 | GEO
Project description:ced2 mutant has reduced expression of NCED3 in response to osmotic stress (PEG, polyethylene glycol) treatments compared to the wild type. Other ABA biosynthesis genes are also greatly reduced in ced2 under osmotic stress. We used microarrays to detail the global programme of gene expression under osmotic stress treatment and identified distinct classes of up/down-regulated genes in the stress pathway. Arabidopsis seedlings were selected at 14 days with 6hr PEG treatment for RNA extraction andhybridization to the Arabidopsis ATH1 Gene expression arrays (Agilent Technologies). From Northern data we have known some of well-known stress responsive gene were significantly changed. We sought to obtain whole gene expression to analysis which genes were affected in the osmotic stress pathway.
2013-02-21 | E-GEOD-44419 | biostudies-arrayexpress