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Effect of NPAP1/C15orf2 overexpression on transcriptome of HEK293 cells

ABSTRACT: To gain insight into the function of Nuclear pore associated protein 1 (NPAP1, formerly C15orf2), we overexpressed NPAP1 in HEK293 cells. We detected no significant difference between NPAP1-expression of induced and uninduced cells in three technical replicates, exept for an approximately 10-fold increase in the NPAP1 transcript itself. This indicates that overexpression of NPAP1 does not change mRNA expression profiles of HEK293 cells. We used microarrays to investigate global gene expression changes depending on the level of NPAP1/C15orf2 We compared NPAP1 overexpressing cells to untreated cells, which do not express detectable amounts of NPAP1 protein, to determine global gene expression changes.

ORGANISM(S): Homo sapiens

SUBMITTER: Ludger Klein-Hitpass 

PROVIDER: E-GEOD-36575 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The imprinted NPAP1/C15orf2 gene in the Prader-Willi syndrome region encodes a nuclear pore complex associated protein.

Neumann Lisa C LC   Markaki Yolanda Y   Mladenov Emil E   Hoffmann Daniel D   Buiting Karin K   Horsthemke Bernhard B  

Human molecular genetics 20120613 18

The Prader-Willi syndrome (PWS) region in 15q11q13 harbours a cluster of imprinted genes expressed from the paternal chromosome only. Whereas loss of function of the SNORD116 genes appears to be responsible for the major features of PWS, the role of the other genes is less clear. One of these genes is C15orf2, which has no orthologues in rodents, but appears to be under strong positive selection in primates. C15orf2 encodes a 1156 amino acid protein with six nuclear localisation sequences. By pr  ...[more]

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