Unknown,Transcriptomics,Genomics,Proteomics

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Effect of FTO overexpression and knockdown on transcriptome of HEK293 cells


ABSTRACT: To gain insight into FTO function, we knocked down and overexpressed FTO in HEK293 cells.Genetrail analyses of expression profiles pointed to the RNA splicing and processing machinery. Intriguingly, using immunocytochemistry and confocal laser scanning microscopy, we observed strong enrichment of FTO in nuclear speckles and - to a lesser extent - in nucleoli, but not in other known nuclear bodies. We also studied RNA samples of Fto knockout and wild type mice with regard to content of methylated and unmethylated nucleosidesand observed that ratios of modified and unmodified uracil and adenine were different depending on the presence of FTO. Taken together, our data suggest that FTO is involved in RNA processing and modification. We used microarrays to investigate global gene expression changes depending on the level of FTO We compared FTO overexpressing and FTO depleted cells to cells with endogeneus level of FTO to determine global gene expression changes.

ORGANISM(S): Homo sapiens

SUBMITTER: Ludger Klein-Hitpass 

PROVIDER: E-GEOD-33870 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

FTO levels affect RNA modification and the transcriptome.

Berulava Tea T   Ziehe Matthias M   Klein-Hitpass Ludger L   Mladenov Emil E   Thomale Jürgen J   Rüther Ulrich U   Horsthemke Bernhard B  

European journal of human genetics : EJHG 20120808 3


A block of single-nucleotide polymorphisms within intron 1 of the FTO (fat mass and obesity associated) gene is associated with variation in body weight. Previous works suggest that increased expression of FTO, which encodes a 2-oxoglutarate-dependent nucleic acid demethylase, leads to increased body weight, although the underlying mechanism has remained unclear. To elucidate the function of FTO, we examined the consequences of altered FTO levels in cultured cells and murine brain. Here we show  ...[more]

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