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Expression data from 10-days old dark grown Arabidopsis seedlings of wild type (Col-0), sps1 line and L17 line.

ABSTRACT: In the present work, experiments were carried out to understand the molecular mechanism underlying phyA’ silencing.The EMS-mutagenesis of phyA’ line, L17, generated suppressor mutations that activate phyA’ locus. Detailed study of one suppressor line, sps1, indicated that methylation of specific CG site within the phyA coding region is critical for maintaining phyA’ silencing. In order to study the possible genomic targets of the putative SPS1 factor, microarray analysis using ATH1 genome array chip was done on sps1, L17 and Col-0. 9 samples in total were analyzed. Each line (WT, sps1, L17) were analyzed in triplicate.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Mariya Khodakovskaya

PROVIDER: E-GEOD-36581 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Site-specific methylation in gene coding region underlies transcriptional silencing of the Phytochrome A epiallele in Arabidopsis thaliana.

Rangani Gulab G Khodakovskaya Mariya M Alimohammadi Mohammad M Hoecker Ute U Srivastava Vibha V

Plant molecular biology 20120401 1-2

DNA methylation in cytosine residues plays an important role in regulating gene expression. Densely methylated transgenes are often silenced. In contrast, several eukaryotic genomes express moderately methylated genes. These methylations are found in the CG context within the coding region (gene body). The role of gene body methylation in gene expression, however, is not clear. The Arabidopsis Phytochrome A epiallele, phyA', carries hypermethylation in several CG sites resident to the coding reg ...[more]

PMID: 22466452

Similar Datasets

Expression data from 10-days old dark grown Arabidopsis seedlings of wild type (Col-0), sps1 line and L17 line.

Project description:In the present work, experiments were carried out to understand the molecular mechanism underlying phyA’ silencing.The EMS-mutagenesis of phyA’ line, L17, generated suppressor mutations that activate phyA’ locus. Detailed study of one suppressor line, sps1, indicated that methylation of specific CG site within the phyA coding region is critical for maintaining phyA’ silencing. In order to study the possible genomic targets of the putative SPS1 factor, microarray analysis using ATH1 genome array chip was done on sps1, L17 and Col-0.

2012-07-01 | GSE36581 | GEO

Expression data from roots and first two leaves of tomato seedlings growing on regular MS medium or MS medium supplemented with multi-wall carbon nanotubes (0, 50, 100, 200 ug/ml) or activated carbon (50 mg/ml)

Project description:To investigate effects of carbon nanotubes (CNTs) om total expression profile of tomato plants we introduced CNTs in MS agar medium in concentrationd 0, 50, 100, 200 ug/ml Objectives for this study included the identification of genes that were up or down-regulated at the transcriptional level in tomato seedlings in response to carnom nanotubes (CNTs). 10 day old root tips and first two leaves of wild type tomato seedlings (cv. Micro-Tom) growing on regular MS agar medium or MS medium supplemented with carbon nanotubes (50, 100, 200 ug/ml) or MS medium supplemented with activated carbon (50 ug/ml) were selected for RNA extraction and hybridization on Affymetrix microarrays.

2010-12-01 | E-GEOD-22803 | biostudies-arrayexpress

Expression data from seedligs of wild type tomato seedlings (control) and InsP 5-ptase tansgenic line (L7) exposed to light stress

Project description:Objectives for this study included the identification of genes that were up or down-regulated at the transcriptional level in tomato InsP 5-ptase transgenic lines exposed to continous light for 7 days (light stress). The RNA was isolated from seedlings of wild-type (WT) and Ins P 5-ptase transgenic line 7 that were germinated on MS medium in a growth chamber with 50 umol m-2 s-1 light intencity at 12 h day-light and 12 h dark for 10 days and then exposed to continous light for 7 days. Affymetrix platform was used for expression analysis.

2015-06-15 | E-GEOD-58521 | biostudies-arrayexpress

Expression analysis of seeds and seedlings of tomato growing on regular MS medium or MS medium supplemented with Helical multi-walled carbon nanotubes (Helical MWCNTs) in concentration 25 ug/ml

Project description:To investigate effects of Helical multi-walled carbon nanotubes (Helical MWCNTs) on total expression profile of tomato seeds and young sedlings we introduced Helical MWCNTs in MS agar medium in concentrationd of 25 ug/ml. Objectives for this study included the identification of genes that were up or down-regulated at the transcriptional level in tomato seeds and seedlings in response to application of Helical MWCNTs Tomato seeds (1 day after placement of medium) and 11-days old seedlings growing on regular MS agar medium or MS medium supplemented with Helical MWCNTs (25 ug/ml) were selected for RNA extraction and hybridization on Affymetrix microarrays.

2016-05-30 | E-GEOD-70842 | biostudies-arrayexpress

Expression data from roots and first two leaves of tomato seedlings growing on regular MS medium or MS medium supplemented with multi-wall carbon nanohorns (25 ug/ml)

Project description:To investigate effects of single-walled carbon nanohorns (SWCNHs) om total expression profile of tomato seeds and plants we introduced SWCNHs in MS agar medium in concentration of 25 ug/ml Objectives for this study included the identification of genes that were up or down-regulated at the transcriptional level in tomato seeds and seedlings in response to SWCNHs Seeds (1 day on medium) and 11-days old seedlings of wild type tomato seedlings (cv. Micro-Tom) growing on regular MS agar medium or MS medium supplemented with carbon nanohorns (25 ug/ml) were selected for RNA extraction and hybridization on Affymetrix microarrays. Keywords: stress responses

2015-01-01 | E-GEOD-57541 | biostudies-arrayexpress

The photoreceptor chaperone FHY1 has an independent role in gene modulation and plant development under far-red light [RNA-Seq]

Project description:To incorporate the far-red light (FR) signal into a strategy for optimizing plant growth, FAR-RED ELONGATED HYPOCOTYL1 (FHY1) mediates the nuclear translocation of the FR photoreceptor phytochrome A (phyA) and facilitates the association of phyA with the promoters of numerous associated genes crucial for the response to environmental stimuli. However, whether FHY1 plays additional roles following FR irradiation remains elusive. Here, by the global identification of FHY1 chromatin association sites through ChIP-seq analysis and by the comparison of FHY1-associated sites with phyA- associated sites, we demonstrated that nuclear FHY1 can either act independently of phyA or act in association with phyA to activate the expression of distinct target genes. We also determined that phyA can act independently of FHY1 in regulating phyA-specific target genes. Furthermore, we determined that the independent FHY1 nuclear pathway is involved in crucial developmental aspects, as in the case of inhibited seed germination under FR during salt-stress. Notably, the differential presence of cis-elements and transcription factors in common and unique FHY1 and/or phyA associated genes are indicative of the complexity of the independent and coordinated FHY1 and phyA pathways. Our study uncovers new aspects of FHY1 function beyond its currently recognized role in phyA-dependent photomorphogenesis. The 35S: GFP-FHY1 fhy1-1 transgenic line and the fhy1-1 mutant were grown under the same light conditions used (D4d+FR3h) for RNA preparation and sequencing. Three biologically replicates were subjected to high-throughput Solexa (Illumina) sequencing.

2014-08-04 | E-GEOD-58082 | biostudies-arrayexpress

The photoreceptor chaperone FHY1 has an independent role in gene modulation and plant development under far-red light [RNA-Seq]

2014-08-04 | GSE58082 | GEO

The photoreceptor chaperone FHY1 has an independent role in gene modulation and plant development under far-red light [ChIP-seq]

2014-08-04 | GSE58083 | GEO

Ruberti A.thaliana Shade Avoidance Response wt and phyA

Project description:Aim of the experiment is the identification of genes differentially regulated in phyA seedlings relative to wild type after prolonged exposure to low R/FR. To this end, gene expression changes were analysed in Arabidopsis wild-type and phyA knock-out mutant seedlings exposed to low R/FR light. The experiment was designed to enable comparison between the different genotypes exposed or not exposed to low R/FR light.

2013-07-29 | E-MEXP-3267 | biostudies-arrayexpress

Arabidopsis phytochrome A directly targets numerous promoters for individualized modulation of genes in wide range of pathways [ChIP-seq]

Project description:The far-red light (FR) photoreceptor phytochrome A (phyA) contains no DNA binding domain but associates with CHS promoter through its chaperon FHY1 and transcription factors to regulate transcription. Here, we performed a genome-wide identification of phyA targets using a combination of phyA ChIP- and RNA-sequencing methods. Our results indicate that the phyA signaling widely impact gene promoters involved in multiple FR-modulated aspects of plant growth. Furthermore, there was an enrichment of hormones- and stresses-responsive elements in the phyA direct target promoters, indicating that a much broader than expected range of transcription factors are involved in the phyA signaling. To verify our hypothesis that phyA regulates genes other than light-responsive ones through interaction with corresponding transcription factors, we examined the phyA action on one of its direct target gene NAC019, encoding an ABAdependent transcription factor. The phyA signaling cascade, consisting of FHY1 and HY5, not only targets two G-boxes on NAC019 promoter for subsequent transcriptional regulation, but also positively coordinates with ABA response for the root elongation inhibition under FR. Our study provides new insights into how plants rapidly fine-tune their growth strategy upon dynamic light environment by escorting photoreceptors to the promoters of hormones- or stresses-responsive genes for individualized modulation.

2014-05-06 | GSE48769 | GEO

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