Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from serum-starved human umbilical vein endothelial cells that have been treated with scrambled (scr) or Map4k4 siRNA to knock down genes of interest 48 hours prior to harvest

ABSTRACT: We identified that knocking down Map4k4 in endothelial cells affected genes associated with the cell cycle, mitosis, and inflammatory genes. We used microarrays to identify genes of interest that are differentially expressed after the addition of scrambled or Map4k4 siRNA. HUVECs were treated with scrambled or siMap4k4. 24 hours later, cells were serum starved overnight. Cells were prepared with this protocol on three different occasions, and total RNA was isolated to provide three biological replicates per sample.

ORGANISM(S): Homo sapiens

SUBMITTER: Michael Czech 

PROVIDER: E-GEOD-78107 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Endothelial Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 Is Critical for Lymphatic Vascular Development and Function.

Roth Flach Rachel J RJ   Guo Chang-An CA   Danai Laura V LV   Yawe Joseph C JC   Gujja Sharvari S   Edwards Yvonne J K YJ   Czech Michael P MP  

Molecular and cellular biology 20160531 12

The molecular mechanisms underlying lymphatic vascular development and function are not well understood. Recent studies have suggested a role for endothelial cell (EC) mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4) in developmental angiogenesis and atherosclerosis. Here, we show that constitutive loss of EC Map4k4 in mice causes postnatal lethality due to chylothorax, suggesting that Map4k4 is required for normal lymphatic vascular function. Mice constitutively lacking EC Map4k  ...[more]

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