Unknown,Transcriptomics,Genomics,Proteomics

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Expression data of pre-stasis cultured HMEC


ABSTRACT: We have generated a large collection of normal human mammary epithelial cell strains from women aged 16 to 91 years, derived from primary tissues, to enable functional and molecular interrogation of aging. We demonstrate in finite-lifespan cultured and uncultured epithelial cells that aging is associated with reduction of myoepithelial cells and with increases in luminal cells expressing keratin 14 and integrin α6, traits that are expressed exclusively in myoepithelial cells in women under 30. We find that changes to the luminal lineage result from age-dependent expansion of multipotent progenitors that bear defects resulting in incompletely differentiated luminal cells. These findings were verified in vivo in normal breast tissues. Myoepithelial cells have been suggested to act as tumor suppressors, and progenitor cells are implicated as the etiological roots of mammary carcinomas. Thus with aging there is a shift in the balance of luminal/myoepithelial lineages, and changes in the functional spectrum of multipotent progenitors, which presages increased potential for malignant transformation. Finite lifespan pre-stasis HMEC from specimens from reduction mammoplasties of 7 patients of different age were collected. The expression data was queried for different biological replicates and at different passages. For strain 240, luminal and myoepithelial cells were collected using flow cytometry.

ORGANISM(S): Homo sapiens

SUBMITTER: Francois Pepin 

PROVIDER: E-GEOD-37485 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Accumulation of multipotent progenitors with a basal differentiation bias during aging of human mammary epithelia.

Garbe James C JC   Pepin Francois F   Pelissier Fanny A FA   Sputova Klara K   Fridriksdottir Agla J AJ   Guo Diana E DE   Villadsen Rene R   Park Morag M   Petersen Ole W OW   Borowsky Alexander D AD   Stampfer Martha R MR   Labarge Mark A MA  

Cancer research 20120502 14


Women older than 50 years account for 75% of new breast cancer diagnoses, and the majority of these tumors are of a luminal subtype. Although age-associated changes, including endocrine profiles and alterations within the breast microenvironment, increase cancer risk, an understanding of the cellular and molecular mechanisms that underlies these observations is lacking. In this study, we generated a large collection of normal human mammary epithelial cell strains from women ages 16 to 91 years,  ...[more]

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