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Expression analysis in parthenogenetic cells through different potency stages

ABSTRACT: Parthenogenetic stem cells were derived from parthenotes, then differentiated to mesenchymal stem cells. These were further reprogrammed to induced pluripotent stem cells, which were finally differentiated to secondary mesenchymal stem cells. Transcriptional analysis was performed at all 4 states of potency to indetify changes in expression stability of partehnogenetic cells. 4 samples for each stage of potency were analyzed, controls were biparental embryonic stem cells and their mesenchymal derivate, as well as biparental induced pluripotent stem cells

ORGANISM(S): Homo sapiens

SUBMITTER: Rita Vassena

PROVIDER: E-GEOD-37711 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression analysis in parthenogenetic cells through different potency stages

Project description:Parthenogenetic stem cells were derived from parthenotes, then differentiated to mesenchymal stem cells. These were further reprogrammed to induced pluripotent stem cells, which were finally differentiated to secondary mesenchymal stem cells. Transcriptional analysis was performed at all 4 states of potency to indetify changes in expression stability of partehnogenetic cells.

2012-05-04 | GSE37711 | GEO

Intrinsic differentiation potential of human postnatal MSC, Mab and MAPC reflected in their transcriptome

Project description:We have employed whole genome microarray expression profiling to identify genes that characterize different populations of human postnatal stem cells and reflect their potency MSC, Mab and MAPC cultures were established from at least three different donors and their transcriptome was assayed using the Agilent 4x44k whole human genome microarray; furthermore, the H9 ESC line from U of Wisconsin and MSC from Lonza were added for comparison MSC = Mesenchymal Stem Cells, MAPC= Multipotent Adult Progenitor Cells, Mab= Mesoangioblasts, ESC= Embryonic Stem Cells

2011-07-12 | E-GEOD-23131 | biostudies-arrayexpress

Tissue-Specific Transcriptional Profiling of Epigenetic Asymmetry in Porcine Parthenogenetic Fetuses1

Project description:Using two complementary approaches, analysis of imprinting of candidate genes by pyrosequencing and expression profiling of parthenogenetic fetuses, we carried a comprehensive survey of genomic imprinting in swine. In the case of imprinted genes where transcription of one of the two parental alleles is silenced, uniparental embryos like parthenotes can be used to measure transcript dosage effects. Using Affymetrix Porcine GeneChip microarrays, four tissues of day 30 fetuses were profiled: brain, fibroblast, liver, and placenta. Keywords: Transcriptional profiling of epigenetic asymmetry in porcine day 30 parthenogenetic and biparental fetal tissues

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Tissue-Specific Transcriptional Profiling of Epigenetic Asymmetry in Porcine Parthenogenetic Fetuses1

2010-05-06 | E-GEOD-10443 | biostudies-arrayexpress

Genome-wide expression analysis of mesenchymal stem cells isolated from clinical donors in response to FGF2 binding heparan sulfate

Project description:Effect of FGF2 binding heparan sulfate on mesenchymal stem cells was studied and found to influence potency. Gene expression profiling was then performed to undertand its response at the transcriptome level.

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MicroRNA expression profiling of Ewing sarcoma cancer stem cells

Project description:We have recently demonstrated that human paediatric mesenchymal stem cells can be reprogrammed toward a Ewing’s sarcoma family tumor (ESFT) cancer stem cell (CSC) phenotype by mechanisms that implicate microRNAs (miRNAs). Here, we show that the miRNA profile of ESFT CSC is shared by embryonic stem cells and CSC from divergent tumor types. We also provide evidence that the miRNA profile of ESFT CSC is the result of reversible disruption of TARBP2-dependent miRNA maturation. Restoration of TARBP2 activity and systemic delivery of synthetic forms of either of two of its targets, miRNA-143 or miRNA-145, inhibited ESFT CSC clonogenicity and tumor growth in vivo. Our observations suggest that CSC self-renewal and tumor initiation may depend on deregulation of TARBP2-dependent miRNA expression. 2 samples of primary Ewing sarcomas, divided into CD133+ and CD133- fractions. One sample of EWS-FLI1 expressing human pediatric mesenchymal stem cells, divided into CD133+ and CD133- fractions. One sample of STA-ET-8.2 cells, divided into CD133+ and CD133- fractions.

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RNA-seq of granulosa-like cells derived from human embryonic stem cell lines from different parental backgrounds, together with their matched undifferentiated samples

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Genome wide expression profiling of mesenchymal stem cells of fetal and adult origin and dermal fibroblasts

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RNA-seq of granulosa-like cells derived from human embryonic stem cell lines from different parental backgrounds

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2023-04-04 | E-MTAB-11660 | biostudies-arrayexpress

MicroRNA expression profiling of Ewing sarcoma spheres vs. adherent cells

Project description:We have recently demonstrated that human paediatric mesenchymal stem cells can be reprogrammed toward a Ewing’s sarcoma family tumor (ESFT) cancer stem cell (CSC) phenotype by mechanisms that implicate microRNAs (miRNAs). Here, we show that the miRNA profile of ESFT CSC is shared by embryonic stem cells and CSC from divergent tumor types. We also provide evidence that the miRNA profile of ESFT CSC is the result of reversible disruption of TARBP2-dependent miRNA maturation. Restoration of TARBP2 activity and systemic delivery of synthetic forms of either of two of its targets, miRNA-143 or miRNA-145, inhibited ESFT CSC clonogenicity and tumor growth in vivo. Our observations suggest that CSC self-renewal and tumor initiation may depend on deregulation of TARBP2-dependent miRNA expression. 2 Ewing sarcoma primary samples: comparison between spheres and derived adherent cells.

2012-06-19 | E-GEOD-31145 | biostudies-arrayexpress

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