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Transcription profiling of mouse undifferentiated and differentiated mammospheres isolated from FVB animaks

ABSTRACT: Mammary gland cells were isolated from 5-8 week old FVB mice and allowed to proliferate as undifferentiated mammospheres in the presence of growth factors (bFGF and EGF). Comparison of the gene expression profiles of undifferentiated mammospheres vs. mammospheres that were allowed to differentiate for 6 days by the removal of growth factors will help to identify genes that are implicated in the maintenance of the mammary gland stem cell compartment. Experiment Overall Design: this experiment include 2 samples and 12 replicates

ORGANISM(S): Mus musculus

SUBMITTER: OGIC Info Ontario Genomics Innovation Centre (OGIC)

PROVIDER: E-GEOD-3777 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene Expression Profiles of Undifferentiated and Differentiated Mammospheres

Project description:Mammary gland cells were isolated from 5-8 week old FVB mice and allowed to proliferate as undifferentiated mammospheres in the presence of growth factors (bFGF and EGF). Comparison of the gene expression profiles of undifferentiated mammospheres vs. mammospheres that were allowed to differentiate for 6 days by the removal of growth factors will help to identify genes that are implicated in the maintenance of the mammary gland stem cell compartment. Keywords: other

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Transcription profiling of mouse mammary glands tumours from PEA3-null and wild-type PEA3 animals

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Transcription profiling of mouse sorted bone marrow samples to determine genes implicated in hematopoetic stem cell identity

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Gene expression profiling of mouse mammary tumorspheres, mammospheres, and mammospheres induced to differentiate

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Gene expression profiling of mouse mammary tumorspheres, mammospheres, and mammospheres induced to differentiate

Project description:Tumorpsheres and mammospheres were used to propagate mouse breast tumor-initiating cells and their normal stem/progenitor counterparts, respectively. Mammospheres induced to differentiate were used to model the more differentiated cells of the mouse mammary gland. We used microarrays to find differentrially expressed genes between tumorspheres, mammospheres, and mammospheres induced to differentiate Primary cells were isolate from either mouse mammary glands (FVB/N) or mouse mammary tumors and placed in stem cell media (MMTV-Neu [N2O2]. Spheres were passaged every 7 days for 3-5 passages and harvested for RNA isolation

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Transcription profiling of mouse pluripotet embyonal carcinoma cells

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Aberrant activation of p53/p66Shc-mInsc axis increases asymmetric divisions and attenuates proliferation of aged mammary stem cells.

Project description:Mammary gland aging is due to aberrant activation of p66Shc, which induces p53/p44 signaling, leading to failure of symmetric divisions, decreased proliferation and reduced regenerative potential of MaSCs. Age-related changes in mammary epithelial cells derived from C57BL/6J WT and p66Shc knockedout mice cultured as stem cell-enriched mammospheres.

2022-12-21 | GSE201253 | GEO

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