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Global Analysis of Carbohydrates Utilization of the Alkaline Hemicellulytic Bacteria Bacillus sp. N16-5

ABSTRACT: Alkaline hemicellulytic bacteria Bacillus sp. N16-5 has abroad substrate spectrum and exhibits great growth ability on complex carbohydrates. In order to get insight into its carbohydrate utilization mechanism, global transcriptional profiles were separately determined for growth on glucose, fructose, mannose, galactose, arabinose, xylose, galactomannan, xylan, pectin and carboxymethyl cellulose by using one-color microarrays. Substrate induced gene expression was measured when culture was grown on glucose, fructose, mannose, galactose, arabinose, xylose, galactomannan, xylan and CMC to mid-logarithmic phase.

ORGANISM(S): Bacillus sp. N16-5

SUBMITTER: song yajian

PROVIDER: E-GEOD-38276 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Global microarray analysis of carbohydrate use in alkaliphilic hemicellulolytic bacterium Bacillus sp. N16-5.

Song Yajian Y Xue Yanfen Y Ma Yanhe Y

PloS one 20130110 1

The alkaliphilic hemicellulolytic bacterium Bacillus sp. N16-5 has a broad substrate spectrum and exhibits the capacity to utilize complex carbohydrates such as galactomannan, xylan, and pectin. In the monosaccharide mixture, sequential utilization by Bacillus sp. N16-5 was observed. Glucose appeared to be its preferential monosaccharide, followed by fructose, mannose, arabinose, xylose, and galactose. Global transcription profiles of the strain were determined separately for growth on six monos ...[more]

PMID: 23326578

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