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Determination of Sigma 54 reglons in Bacillus thuringiensis HD73 strain

ABSTRACT: To determine Sigma 54 (SigL) reglons in Bacillus thuringiensis HD73 strain, A sigLmutant, HD(M-NM-^TsigL::kan), was constructed with insertion of kanamycin resistance gene cassete. We have employed whole genome microarray expression profiling as a discovery platform to identify the difference of gene expression between mutant and wild-type strains. 2 ml samples were separately harvested from B. thuringiensis HD73 and HD(M-NM-^TsigL::kan) strains grown in SchaefferM-bM-^@M-^Ys sporulation medium (SSM) at stages T7 of stationary phase (7 hours after the end of the exponential phase). Three independent repeats were performed for each stain.

ORGANISM(S): Bacillus thuringiensis

SUBMITTER: Fuping Song

PROVIDER: E-GEOD-48410 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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