Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Gene expression of mouse embryonic gonad [Nanos3]

ABSTRACT: To identify germ cell- and somatic cell-specific gene expression profiles, we performed expression microarray analysis of the mouse gonads of the Nanos3+/-, Nanos3-/- female and male embryos from E12.5 to E15.5. Biological duplicates were examined at each stage, genotype, and sex for each experiment.

ORGANISM(S): Mus musculus

SUBMITTER: Rie Saba

PROVIDER: E-GEOD-38297 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Nodal/activin signaling promotes male germ cell fate and suppresses female programming in somatic cells.

Wu Quan Q Kanata Kohei K Saba Rie R Deng Chu-Xia CX Hamada Hiroshi H Saga Yumiko Y

Development (Cambridge, England) 20121205 2

Testicular development in the mouse is triggered in somatic cells by the function of Sry followed by the activation of fibroblast growth factor 9 (FGF9), which regulates testicular differentiation in both somatic and germ cells. However, the mechanism is unknown. We show here that the nodal/activin signaling pathway is activated in both male germ cells and somatic cells. Disruption of nodal/activin signaling drives male germ cells into meiosis and causes ectopic initiation of female-specific gen ...[more]

PMID: 23221368

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Project description:To identify genes that are downstream of gonadal hormones and that control dimorphic behaviors, we used a MEEBO array platform to profile gene expression of adult male and female hypothalamus against a whole brain reference sample. The experimental design allowed us to identify genes that are upreguated in the hypothalamus compared to the whole brain and are dimorphically expressed between the two sexes. In situ hybridization of candiate genes were carried out to validate the dimorphic expression of these genes in the hypothalamus. Array results were used to create a list of genes to screen by in situ hybridization. For each normalization method, a list of genes that were upregulated in the male or the female was created, and a gene had to be on a set number of these individual normalization lists in order to be considered for screening. A similar method was used to create a list of hypothalamic upregulated genes. The final screening list was comprised of genes that were upregulated in the male or female as well as in the hypothalamus. If in situ hybridization proved that the gene was upregulated in the male or female brain, we concluded that that transcript was differentially expressed. No one normalization method was weighted over another, and the array results were used to create a screening list for in situ hybridizations. Hypothalami from 4 adult animals of each sex were microdissected and pooled for each experimental sample and the whole brain plus pituitary from one male and one female were pooled to provide the reference sample. Each set of samples (male and female) was hybridized to two arrays to provide two technical replicates for each set of samples taken. A total of three sets of samples were taken (three biological replicates). For the "_amp" Samples, source (hypothalamus and whole brain) mRNA was amplified using T7 based method. For this set, data were not normalized, rather the raw intensities were used to calculate ratios. Candidate genes from this study was also included in final list in situ screening.

Dataset Information

Gene expression of mouse embryonic gonad [Nanos3]

Publications

Nodal/activin signaling promotes male germ cell fate and suppresses female programming in somatic cells.

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