Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Deeply conserved gene-regulatory functions of carboxy-terminal domains in dictyostelid C-module-binding factors

ABSTRACT: We describe the gene expression pattern of a Dictyostelium discoideum mutant depleted in the C-module-binding factor (CbfA) and autonomous gene-regulatory activities of CbfA carboxy-terminal domains (CbfA-CTDs) Comparison of gene expression in growing cells of a CbfA mutant compared to wild-type

ORGANISM(S): Dictyostelium discoideum

SUBMITTER: Marco Groth

PROVIDER: E-GEOD-38418 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Dictyostelium discoideum O-Ser-fucome from WT and SPY-KO developing cells (slugs)

Project description:Dictyostelium discoideum O-Ser-fucosylated proteins from WT and SPY-KO developing cells (slugs) were immunoprecipitated using anti O-Ser fucose polyclonal antibodies in 3 Biological Replicates, with 3 Technical Replicates each, by comparing AX3 (WT strain) against SPY-KO (mutant, deficient in O-fucosylation).

2025-01-30 | PXD056903 | Pride

Dictyostelium discoideum O-Thr-fucome from WT and SPY-KO developing cells (slugs)

Project description:Dictyostelium discoideum O-Thr-fucosylated proteins from WT and SPY-KO developing cells (slugs) were immunoprecipitated using anti O-Thr fucose polyclonal antibodies in 3 Biological Replicates, with 3 Technical Replicates each, by comparing AX3 (WT strain) against SPY-KO (mutant, deficient in O-fucosylation).

2025-01-30 | PXD056866 | Pride

Dictyostelium discoideum O-Ser-fucome from WT and SPY-KO vegetative cells

Project description:Dictyostelium discoideum O-Ser-fucosylated proteins from WT and SPY-KO vegetative cells were immunoprecipitated using anti O-Ser fucose polyclonal antibodies in 3 Biological Replicates, with 3 to 6 Technical Replicates each, by comparing AX3 (WT strain) against SPY-KO (mutant, deficient in O-fucosylation).

2025-01-30 | PXD056853 | Pride

Dictyostelium discoideum O-Thr-fucome from WT and SPY-KO vegetative cells

Project description:Dictyostelium discoideum O-Thr-fucosylated proteins from WT and SPY-KO vegetative cells were immunoprecipitated using anti O-Thr fucose polyclonal antibodies in 3 Biological Replicates, with 3 to 6 Technical Replicates each, by comparing AX3 (WT strain) against SPY-KO (mutant, deficient in O-fucosylation).

2025-01-30 | PXD056857 | Pride

Dictyostelium discoideum O-fucome from WT, SPY-OE, and SPY-KO vegetative cells

Project description:Dictyostelium discoideum O-Ser and O-Thr fucosylated proteins from WT, SPY-OE and SPY-KO vegetative cells were immunoprecipitated using anti O-Ser and anti O-Thr fucose polyclonal antibodies in 3 Technical Replicates, by comparing AX3 (WT strain) against SPY-KO (mutant, deficient in O-fucosylation), and SPY_OE (mutant, SPY overexpressor strain).

2025-01-30 | PXD057018 | Pride

Deeply conserved gene-regulatory functions of carboxy-terminal domains in dictyostelid C-module-binding factors

Project description:We describe the gene expression pattern of a Dictyostelium discoideum mutant depleted in the C-module-binding factor (CbfA) and autonomous gene-regulatory activities of CbfA carboxy-terminal domains (CbfA-CTDs)

2012-06-03 | GSE38418 | GEO

abc transporters in Dictyostelium discoideum development

Project description:ATP-binding cassette (ABC) transporters can translocate a broad spectrum of molecules across the cell membrane including physiological cargo and toxins. ABC transporters are known for the role they play in resistance towards anticancer agents in chemotherapy of cancer patients. There are 68 ABC transporters annotated in the genome of the social amoeba Dictyostelium discoideum. We have characterized more than half of these ABC transporters through a systematic study of mutations in their genes. We have analyzed morphological and transcriptional phenotypes for these mutants during growth and development and found that most of the mutants exhibited rather subtle phenotypes. A few of the genes may share physiological functions, as reflected in their transcriptional phenotypes. Since most of the abc-transporter mutants showed subtle morphological phenotypes, we utilized these transcriptional phenotypes to identify genes that are important for development by looking for transcripts whose abundance was unperturbed in most of the mutants. We found a set of 668 genes that includes many validated D. discoideum developmental genes. We have also found that abcG6 and abcG18 may have potential roles in intercellular signaling during terminal differentiation of spores and stalks. Transcriptional phenotyping during development of abc transporter mutants in Dictyostelium discoideum

2013-06-14 | E-GEOD-45555 | biostudies-arrayexpress

Identification of target genes of Cud-type transcription factor, spaA, in a Dictyostelid social amoebae by ChIP-seq

Project description:spaA is a Cud-type transcription factor that is essential for spore cell differentiation of Dictyostelium discoideum, a social amoeba. ChIP-seq was performed to identify spaA target genes.

2018-05-31 | E-MTAB-6000 | biostudies-arrayexpress

RNA-Seq data of terminal differentiated cells in Dictyostelium discoideum

Project description:The intent of the experiment was to gather transcriptomic data from terminally differentiated cell types --spore, stalk, and cup cells-- in a simple multicellular model system, Dictyostelium discoideum, in order to characterize the cell-type specific gene expression patterns. Specifically, we dissociated and collected each cell type from the fruiting bodies of D. discoideum at 24 hours of development. We also collected exponentially growing vegetative cells of D. discoideum.

2019-01-31 | E-MTAB-7215 | biostudies-arrayexpress

The large GTPase Sey1/atlastin mediates lipid droplet- and FadL-dependent intracellular fatty acid metabolism of Legionella pneumophila

Project description:The facultative intracellular bacterium Legionella pneumophila employs the Icm/Dot type IV secretion system (T4SS) to replicate in a unique membrane-bound compartment, the Legionella-containing vacuole (LCV). The ER-resident large fusion GTPase Sey1/atlastin-3 promotes remodeling and expansion of LCVs, and the GTPase is also implicated in the formation of ER-derived lipid droplets (LDs). Here we reveal that LCVs intimately interact with palmitate-induced LDs in Dictyostelium discoideum amoeba. Comparative proteomics of LDs isolated from the D. discoideum parental strain Ax3 or sey1 revealed 192 differentially produced proteins, of which 7 or 22 were exclusively detected in LDs isolated from strain Ax3 or sey1, respectively. Using dually fluorescence-labeled amoeba producing the LCV marker P4C-GFP or AmtA-GFP and the LD marker mCherry-perilipin, we show that Sey1 and the L. pneumophila Icm/Dot T4SS as well as the effector LegG1 promote LCV-LD interactions. In vitro reconstitution of the LCV-LD interactions using purified LCVs and LDs from D. discoideum Ax3 or sey1 revealed that Sey1 and GTP promote this process. The LCV-LD interactions were impaired forsey1-derived LDs, suggesting that Sey1 regulates LD traits. Palmitate promoted the growth of L. pneumophila in D. discoideum Ax3 but not in sey1 and of wild-type but not mutant bacteria lacking a homologue of the E. coli fatty acid transporter FadL. Finally, isotopologue profiling indicated that intracellular L. pneumophila metabolizes 13C-palmitate, and its catabolism was reduced in D. discoideum sey1 and L. pneumophila fadL. Taken together, our results reveal that Sey1 mediates LD- and FadL-dependent fatty acid metabolism of intracellular L. pneumophila

2023-06-07 | PXD038200 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data