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Live cell monitoring of periodic gene expression in synchronous human cells identifies forkhead genes involved in cell cycle control

ABSTRACT: We report the genome wide DNA binding patterns of wild type FOXK1 in asynchronous HeLa cells using chromatin precipitation followed by high-throughput sequencing (ChIP-seq). We find that FOXK1 is bound to the promoter of a number of cell cycle genes including the E2F binding partner TFDP1. This study is the first reported study of FOXK1 binding on a genome-wide scale in HeLa cells. Chromatin precipitation

ORGANISM(S): Homo sapiens

SUBMITTER: Gavin Grant

PROVIDER: E-GEOD-39138 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Live-cell monitoring of periodic gene expression in synchronous human cells identifies Forkhead genes involved in cell cycle control.

Grant Gavin D GD Gamsby Joshua J Martyanov Viktor V Brooks Lionel L George Lacy K LK Mahoney J Matthew JM Loros Jennifer J JJ Dunlap Jay C JC Whitfield Michael L ML

Molecular biology of the cell 20120627 16

We developed a system to monitor periodic luciferase activity from cell cycle-regulated promoters in synchronous cells. Reporters were driven by a minimal human E2F1 promoter with peak expression in G1/S or a basal promoter with six Forkhead DNA-binding sites with peak expression at G2/M. After cell cycle synchronization, luciferase activity was measured in live cells at 10-min intervals across three to four synchronous cell cycles, allowing unprecedented resolution of cell cycle-regulated gene ...[more]

PMID: 22740631

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