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Transcriptomics of Bacillus thuringiensis Strain CT-43.

ABSTRACT: We investigated the gene expression and metabolic regulatory mechanisms associated with the high-level accumulation of ICPs by performing the transcriptomics analysis of B. thuringiensis strain CT-43, using Illumina high throughout sequencing (RNA-seq) technique. The bacterial cells were collected at the time points of 7 h, 9 h, 13 h and 22 h for the whole-genome transcriptomics, respectively.

ORGANISM(S): Bacillus thuringiensis

SUBMITTER: Wang Jieping

PROVIDER: E-GEOD-39479 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The metabolic regulation of sporulation and parasporal crystal formation in Bacillus thuringiensis revealed by transcriptomics and proteomics.

Wang Jieping J Mei Han H Zheng Cao C Qian Hongliang H Cui Cui C Fu Yang Y Su Jianmei J Liu Ziduo Z Yu Ziniu Z He Jin J

Molecular & cellular proteomics : MCP 20130212 5

Bacillus thuringiensis is a well-known entomopathogenic bacterium used worldwide as an environmentally compatible biopesticide. During sporulation, B. thuringiensis accumulates a large number of parasporal crystals consisting of insecticidal crystal proteins (ICPs) that can account for nearly 20-30% of the cell's dry weight. However, the metabolic regulation mechanisms of ICP synthesis remain to be elucidated. In this study, the combined efforts in transcriptomics and proteomics mainly uncovered ...[more]

PMID: 23408684

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