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Gene expression profiles in black soybean sprouts: 0.5 cm long vs 5 cm long

ABSTRACT: To explore the mechanism underlying antioxidant activity of extracts from black soybean sprouts 0.5 cm long, Agilent-016772 G. max (Soybean) Oligo Microarray 4x44K was used to compare mRNA expression between the black soybean sprouts 0.5 cm long (n=4) and the black soybean sprouts 5 cm long (n=4). GO term enrichment analysis showed ten up-regulated genes (BE823689.1_567, GMFL01-02-F14-R_381, GMFL01-03-G22-R_364, GMFL01-14-M12-R_553, GMFL01-51-M23-R_265, AW757007.1_297, AW761420.1_260, BI788389.1_501, BQ273202.1_332 and GMFL01-10-I14-F_701) in the 0.5 cm seedlings were associated with response to oxidative stress. qRT-PCR assay confirmed the up-regulation of these ten genes in sprouts 0.5 cm long. In conclusion, these ten genes may contribute to antioxidant activity of sprout extract. Gene expressions in black soybean sprouts were measured using Agilent-016772 G. max (Soybean) Oligo Microarray 4x44K. Four independent experiments were performed in each group using different sprout sample.

ORGANISM(S): Glycine max

SUBMITTER: Hong Weiguo

PROVIDER: E-GEOD-40002 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression profiles in black soybean sprouts: 0.5 cm long vs 5 cm long

Project description:To explore the mechanism underlying antioxidant activity of extracts from black soybean sprouts 0.5 cm long, Agilent-016772 G. max (Soybean) Oligo Microarray 4x44K was used to compare mRNA expression between the black soybean sprouts 0.5 cm long (n=4) and the black soybean sprouts 5 cm long (n=4). GO term enrichment analysis showed ten up-regulated genes (BE823689.1_567, GMFL01-02-F14-R_381, GMFL01-03-G22-R_364, GMFL01-14-M12-R_553, GMFL01-51-M23-R_265, AW757007.1_297, AW761420.1_260, BI788389.1_501, BQ273202.1_332 and GMFL01-10-I14-F_701) in the 0.5 cm seedlings were associated with response to oxidative stress. qRT-PCR assay confirmed the up-regulation of these ten genes in sprouts 0.5 cm long. In conclusion, these ten genes may contribute to antioxidant activity of sprout extract.

2012-08-10 | GSE40002 | GEO

Glycine max

Project description:Gene expression profiles in black soybean sprouts: 0.5 cm long vs 5 cm long

| PRJNA172251 | ENA

Comparative proteomics analysis of soybean sprouts that treated with deionized water or with 6 mM Ca2+

Project description:Soybean sprout, a kind of year-round vegetable in Asia, is perceived as a part of a healthy diet. We found that supplemental Ca2+ could increase soybean sprout yield and improve its nutrition qualities. Ca2+-treated sprouts had higher yield than water-treated ones. Metabolism of selected anti-nutritional factors and bioactive substances in soybean sprouts was strengthened by Ca2+. To investigate the role of Ca2+ in soybean during germination, proteomic changes were analyzed. Total protein from soybean sprouts that treated with deionized water or with 6 mM Ca2+ were analyzed.

2016-04-26 | PXD003359 | Pride

Expression data from the seed coats of black (iRT) and brown (irT) soybean variant for alleles of the R locus

Project description:The seed coat of black (iRT) soybean with the dominant R allele begins to accumulate cyanic pigments at the transition stage of seed development (300 – 400 mg fresh seed weight), whereas the brown (irT) nearly-isogenic seed coat with the recessive r allele lacks cyanic pigments at all stages of seed development. We used microarrays to determine global gene expression differences between black (iRT) and brown (irT) soybean seed coats at the transition stage of seed development (300 – 400 mg fresh seed weight). To identify the complete set of gene transcripts that are differentially expressed between the seed coats of black (iRT) and brown (irT) Clark isolines, seed coats were dissected at the transition stage of seed development (300 – 400 mg fresh seed weight) for microarray analysis using the Affymetrix Soybean GeneChip. To ensure seed coats were of the same stage of development, the days post anthesis, pod length, pod color, embryo morphology, and transcript levels of the developmental marker gene Gm-r1083-1191, a putative cutin biosynthesis gene, and DFR1 were ensured to be equivalent between black (iRT) and brown (irT) isolines.

2011-08-22 | E-GEOD-26208 | biostudies-arrayexpress

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Project description:We want to obtained miRNA-seq from 4 day-old broccoli sprouts by deep sequencing and identified the binding sites of the those miRNAs in human target genes. Meanwhile, we extracted the human target genes from published paper that were regulated by broccoli and compared those genes with the predicted human targets of 4-day broccoli sprouts.

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Sprouting process in potato tuber

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2020-07-28 | E-MTAB-7137 | biostudies-arrayexpress

Soybean root hair cell response to Bradyrhizobium japonicum inoculation

Project description:Soybean root hair transcriptional response to their inoculation by the symbiotic bacteria B. japonicum involved in soybean nodulation. We used the first generation of an Affymetrix microarray to quantify the abundance of the transcripts from soybean root hair cells inoculated and mock-inoculated by B. japonicum. This experiment was performed on a time-course from 6 to 48 hours after inoculation. Soybean seeds were sowed on sterile agar medium and grown for 3 days in a growth chamber before being treated with H2O (mock-inoculated) or B. japonicum (inoculated). Soybean root hair cells were isolated at different time points (6hr, 12hr, 18hr, 24hr, 36hr, 48hr) after treatment. For each time point and condition, 3 or 4 independent biological replicates were produced.

2010-09-16 | E-GEOD-24145 | biostudies-arrayexpress

Ayu macrophages at 3.5 hours, 0, 0.5 or 5 ug/ml Leukocyte cell-derived chemotaxin 2 (LECT2), gene expression array, three independent biological replicates

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2012-08-21 | E-GEOD-40221 | biostudies-arrayexpress

Expression data from salinity treated and control soybean

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