Dataset Information

Systematic Discovery of Deeply Conserved Plant Protein Complexes, DSSO-crosslinked soy (Glycine max)

ABSTRACT: The associated files are mass spec data from size exclusion chromatography fractions that were subsequently crosslinked with DSSO. The starting material was a native extract prepared from soy sprouts (Glycine max). The mass spectrometry used a data-dependent MS2-MS3 method to identify crosslinks.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Glycine Max

TISSUE(S): Shoot

SUBMITTER: Ophelia Papoulas

LAB HEAD: Edward M. Marcotte

PROVIDER: PXD013704 | Pride | 2019-10-28

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Soy_SEC_DSSO_02a_07192018-3.msf	Msf
	Soy_SEC_DSSO_02a_07192018-3.pep.xml	Pepxml
	Soy_SEC_DSSO_02a_07192018.raw	Raw
	Soy_SEC_DSSO_02b_08152018.msf	Msf
	Soy_SEC_DSSO_02b_08152018.pep.xml	Pepxml

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Project description:The transition metal tungsten (W) finds increasing application in military, aviation and household appliance industry, opening new paths for the heavy metal into the environment. Since tungsten shares certain chemical properties with the essential plant micro nutrient molybdenum, it is proposed to inhibit enzymatic activity of molybdoenzymes by replacing the Mo-ion bound to the co-factor. However, recent studies suggest the inhibition of enzymatic activity might not be the only effect W has on plants and that, much like other heavy metals, tungsten exerts toxicity on its own. Still, our understanding of the mechanisms behind the apparent phytotoxicity remains limited.This study investigates the effects of W on growth, nutrient levels (ionome, ICP-MS), starch levels and nitrogen nutrition (IRMS, enzyme activity assays) as well as root and nodule proteome (LC-MS/MS) of Glycine max cv. Primus. Plants were inoculated with Bradyrhizobium japonicum and grown in a semi hydroponic set up using three different tungsten concentrations (zero, 0.1 mM and 0.5 mM). To identify possible benefits of a functional bacterial symbiosis on W induced stress response, two different environmental growth conditions, one with suppressed N-fixation, supplied with Nitrate (10 mM KNO3) and one solely relying on symbiotic N-fixation (zero KNO3), were applied. Glycine max was able to take up considerable amounts of W (703 ± 136 mg kg-1). However, high W resulted in a strong reduction of shoot biomass of both N regimes and of root biomass (N fed only). Irrespective of N regime, NR activity and total N decreased with increasing W concentrations. Similarly, nitrogenase precursor levels as well as N2-fixation, nodule fixation activity (mg N g-1 nodule dry weight) and nodulation were reduced, indicating that nitrogenase synthesis and activity were negatively affected by W stress. However, along a N fix nodule specific induction of the secondary and phytohormone metabolism, it appears that N2 fixation of remaining nodules was not as severely affected by increasing levels of tungsten than nitrate reduction. Besides N metabolism, plants exhibited an imbalance in nutrient and a failure of carbon metabolic pathways accompanied by an accumulation of starch at high tungsten concentrations, independent of N-regime. Proteomic data demonstrated that the response to high W concentrations is independent of nodule functionality and dominated by several peroxidases and other general stress related proteins. Based on an evaluation of several W responsive proteotypic peptides, we identified a set of protein markers of W stress and possible targets for improved stress tolerance.

Dataset Information

Systematic Discovery of Deeply Conserved Plant Protein Complexes, DSSO-crosslinked soy (Glycine max)

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