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MiRNA profiles of brain glioma stem cells and normal neural stem cells

ABSTRACT: A human microarray comprising 1658 human genome probes was used to evaluate the specific expression of miRNA between brian glioma stem cells (GSC) and normal neural stem cells(NSC). 2 total RNA samples are analyzed, brian glioma stem cells (GSC) and normal neural stem cells(NSC). cell type: brain glioma stem cells:GSC_1, GSC_2, GSC_3 ; cell line: normal neural stem cells: NSC_1, NSC_2, NSC_3 biological replicate: NSC_1, NSC_2, NSC_3; biological replicate: GSC_1, GSC_2, GSC_3

ORGANISM(S): Homo sapiens

SUBMITTER: Shuang Liu

PROVIDER: E-GEOD-41032 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Regulatory roles of miRNA in the human neural stem cell transformation to glioma stem cells.

Liu Shuang S Yin Feng F Zhang Jianning J Wicha Max S MS Chang Alfred E AE Fan Wenhong W Chen Ling L Fan Ming M Li Qiao Q

Journal of cellular biochemistry 20140801 8

To investigate the expressional alternation of microRNAs (miRNA) during the malignant transformation and development of human glioma, we measured miRNA expression profile as well as mRNA expression profile in normal human neural stem cells (hNSCs) and human glioma stem cells (hGSCs). We found 116 miRNA up-regulated and 62 miRNA down-regulated in GSCs. On the other hand, we identified 1,372 mRNA down-regulated, and 1,501 mRNA up-regulated in GSCs compared to those in NSCs. We then analyzed the pa ...[more]

PMID: 24519663

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