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Effect of Bach2 overexpression in BCL6+/+ and -/- BCR-ABL1 transformed pre-B cells

ABSTRACT: The aim was to investigate how BCL6 genotype affects Bach2 dependent gene expression changes. We compared gene expression profiles of BCL6+/+ and BCL6-/- BCR-ABL1 transformed pre-B cells after inducible overexpression of Bach2.

ORGANISM(S): Mus musculus

SUBMITTER: Markus Müschen

PROVIDER: E-GEOD-41042 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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BACH2 mediates negative selection and p53-dependent tumor suppression at the pre-B cell receptor checkpoint.

Swaminathan Srividya S Huang Chuanxin C Geng Huimin H Chen Zhengshan Z Harvey Richard R Kang Huining H Ng Carina C Titz Björn B Hurtz Christian C Sadiyah Mohammed Firas MF Nowak Daniel D Thoennissen Gabriela B GB Rand Vikki V Graeber Thomas G TG Koeffler H Phillip HP Carroll William L WL Willman Cheryl L CL Hall Andrew G AG Igarashi Kazuhiko K Melnick Ari A Müschen Markus M

Nature medicine 20130714 8

The B cell-specific transcription factor BACH2 is required for affinity maturation of B cells. Here we show that Bach2-mediated activation of p53 is required for stringent elimination of pre-B cells that failed to productively rearrange immunoglobulin VH-DJH gene segments. After productive VH-DJH gene rearrangement, pre-B cell receptor signaling ends BACH2-mediated negative selection through B cell lymphoma 6 (BCL6)-mediated repression of p53. In patients with pre-B acute lymphoblastic leukemia, ...[more]

PMID: 23852341

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