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Genome-wide analysis of the SNAPc complex [Illumina array]

ABSTRACT: The small nuclear RNA (snRNA)-activating protein complex (SNAPc) is a basal transcription factor that mediates the transcriptional activation of snRNAs. Here, we describe the genome-wide occupancy of the SNAPC1_and SNAPC4 subunits of SNAPc. While the SNAPC4 occupancy was in accord with the role for SNAPc in snRNA transcription, SNAPC1_displayed a broader genomic profile mirroring that of RNA polymerase II at highly active protein-coding genes. Our functional analysis revealed a role for SNAPC1_in regulation of both basal and activator-induced transcription of protein-coding genes. These studies expand the role for SNAPC1_beyond its regulation of snRNA transcription. EGF stimulation of Hela cells, transfected with a control sh (scr) or a SNAPC1_sh

ORGANISM(S): Homo sapiens

SUBMITTER: Alessandro Gardini

PROVIDER: E-GEOD-41528 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Requirement for SNAPC1 in transcriptional responsiveness to diverse extracellular signals.

Baillat David D Gardini Alessandro A Cesaroni Matteo M Shiekhattar Ramin R

Molecular and cellular biology 20120910 22

Initiation of transcription of RNA polymerase II (RNAPII)-dependent genes requires the participation of a host of basal transcription factors. Among genes requiring RNAPII for transcription, small nuclear RNAs (snRNAs) display a further requirement for a factor known as snRNA-activating protein complex (SNAPc). The scope of the biological function of SNAPc and its requirement for transcription of protein-coding genes has not been elucidated. To determine the genome-wide occupancy of SNAPc, we pe ...[more]

PMID: 22966203

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Genome-wide analysis of the SNAPc complex [ChIP-Seq]

Project description:The small nuclear RNA (snRNA)-activating protein complex (SNAPc) is a basal transcription factor that mediates the transcriptional activation of snRNAs. Here, we describe the genome-wide occupancy of the SNAPC1 and SNAPC4 subunits of SNAPc. While the SNAPC4 occupancy was in accord with the role for SNAPc in snRNA transcription, SNAPC1 displayed a broader genomic profile mirroring that of RNA polymerase II at highly active protein-coding genes. Our functional analysis revealed a role for SNAPc1 in regulation of both basal and activator-induced transcription of protein-coding genes. These studies expand the role for SNAPC1 beyond its regulation of snRNA transcription. Comparison of the genome wide profiles of different SNAPc subunits and RNAPII in human cells

2012-09-28 | E-GEOD-37403 | biostudies-arrayexpress

Genome-wide analysis of the SNAPc complex [ChIP-Seq]

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Genome-wide analysis of the SNAPc complex [Illumina array]

Project description:The small nuclear RNA (snRNA)-activating protein complex (SNAPc) is a basal transcription factor that mediates the transcriptional activation of snRNAs. Here, we describe the genome-wide occupancy of the SNAPC1_and SNAPC4 subunits of SNAPc. While the SNAPC4 occupancy was in accord with the role for SNAPc in snRNA transcription, SNAPC1_displayed a broader genomic profile mirroring that of RNA polymerase II at highly active protein-coding genes. Our functional analysis revealed a role for SNAPC1_in regulation of both basal and activator-induced transcription of protein-coding genes. These studies expand the role for SNAPC1_beyond its regulation of snRNA transcription.

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Genome-wide analysis of the SNAPc complex

Project description:This SuperSeries is composed of the following subset Series: GSE37403: Genome-wide analysis of the SNAPc complex [ChIP-Seq] GSE41528: Genome-wide analysis of the SNAPc complex [Illumina array] Refer to individual Series

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Genome-wide analysis of BRCA1 and PALB2 [TNF-alpha stimulation]

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