Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Gregor Gilfillan
PROVIDER: E-GEOD-42147 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Gilfillan Gregor D GD Hughes Timothy T Sheng Ying Y Hjorthaug Hanne S HS Straub Tobias T Gervin Kristina K Harris Jennifer R JR Undlien Dag E DE Lyle Robert R
BMC genomics 20121121
<h4>Background</h4>Chromatin immunoprecipitation coupled with high-throughput DNA sequencing (ChIP-seq) offers high resolution, genome-wide analysis of DNA-protein interactions. However, current standard methods require abundant starting material in the range of 1-20 million cells per immunoprecipitation, and remain a bottleneck to the acquisition of biologically relevant epigenetic data. Using a ChIP-seq protocol optimised for low cell numbers (down to 100,000 cells/IP), we examined the perform ...[more]