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Unknown,Transcriptomics,Genomics,Proteomics

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Runx3 function in CD4+ splenic dendritic cells

ABSTRACT: CD4+ dendritic cells are part of the innate immunity essential for priming and activating of CD4+ T cells To identify Runx3 responsive genes CD4+ dendritic cells were sorted from freshly isolated macs enriched splenic DCs taken from 6 weeks old mice. Six samples from six mice were sorted and analyzed where in each littermates pair consisted of a control and Runx3 KO.

ORGANISM(S): Mus musculus

SUBMITTER: Yoram Groner

PROVIDER: E-GEOD-48589 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptional reprogramming of CD11b+Esam(hi) dendritic cell identity and function by loss of Runx3.

Dicken Joseph J Mildner Alexander A Leshkowitz Dena D Touw Ivo P IP Hantisteanu Shay S Jung Steffen S Groner Yoram Y

PloS one 20131015 10

Classical dendritic cells (cDC) are specialized antigen-presenting cells mediating immunity and tolerance. cDC cell-lineage decisions are largely controlled by transcriptional factor regulatory cascades. Using an in vivo cell-specific targeting of Runx3 at various stages of DC lineage development we show that Runx3 is required for cell-identity, homeostasis and function of splenic Esam(hi) DC. Ablation of Runx3 in DC progenitors led to a substantial decrease in splenic CD4(+)/CD11b(+) DC. Combin ...[more]

PMID: 24204843

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