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Comparative transcriptome analysis of Th22 and Th17 cells

ABSTRACT: Microarray was used to delineate the global gene expression profile underlying the specific developmental program of two divergent antigen-specific T helper subsets (Th22 versus Th17) by identifying upregulation or downregulation of key lineage-determining transcription factors, cytokines, chemokines and other genes that govern their functional attributes. To identify factors that might distinguish the Th22 and Th17 developmental programs, comparative global transcriptome analysis between these 2 subsets was performed. Naïve splenic CD4+ T cells from OT-II-transgenic mice were isolated and grown in vitro under T-helper lineage-specific conditions in the presence of cognate antigen (ovalbumin) to identify their distinctive global gene-regulation profiles.

ORGANISM(S): Mus musculus

SUBMITTER: CASEY WEAVER

PROVIDER: E-GEOD-42332 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Th22 cells are an important source of IL-22 for host protection against enteropathogenic bacteria.

Basu Rajatava R O'Quinn Darrell B DB Silberger Daniel J DJ Schoeb Trenton R TR Fouser Lynette L Ouyang Wenjun W Hatton Robin D RD Weaver Casey T CT

Immunity 20121129 6

Interleukin-22 (IL-22) is central to host protection against bacterial infections at barrier sites. Both innate lymphoid cells (ILCs) and T cells produce IL-22. However, the specific contributions of CD4(+) T cells and their developmental origins are unclear. We found that the enteric pathogen Citrobacter rodentium induced sequential waves of IL-22-producing ILCs and CD4(+) T cells that were each critical to host defense during a primary infection. Whereas IL-22 production by ILCs was strictly I ...[more]

PMID: 23200827

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