Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Transcription profiling of mouse spleen CD4+ CD44Low CD62LHigh cells stimulated with anti-CD3 or anti-CD28 in the presence or absence of hTGFb-1 and mouse IL-6


ABSTRACT: Mouse spleen CD4+CD44LowCD62LHigh cells were stimulated with 2ug/ml anti-CD3(eBioscience), 2ug/ml anti-CD28 (eBioscience) in the presence (Th17 condition) or absence (Th0 condition) of hTGFb-1 (1ng/ml eBioscience), mouse IL-6(10ng/ml eBioscience). Cells were collected after 24 hourÕs culture using RNAeasy columns (Qiagen) for total RNA isolation. Isolated total RNA from Th0 and Th17 conditioned cells were submitted for the microarray gene transcription comparison analysis using Affymetrix Mouse 430A 2.0 array chips.

ORGANISM(S): Mus musculus

SUBMITTER: Hulin Jin Kuchroo 

PROVIDER: E-TABM-583 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2014-05-29 | E-GEOD-54627 | biostudies-arrayexpress
2016-07-30 | E-GEOD-80834 | biostudies-arrayexpress
2017-05-03 | E-GEOD-67185 | biostudies-arrayexpress
2022-02-02 | GSE179625 | GEO
| PRJEB75221 | ENA
2013-12-20 | E-GEOD-53514 | biostudies-arrayexpress
2013-01-29 | E-GEOD-42332 | biostudies-arrayexpress
2014-08-04 | E-GEOD-57500 | biostudies-arrayexpress
2018-02-13 | GSE105056 | GEO
2011-11-01 | E-MTAB-447 | biostudies-arrayexpress