Transcription profiling of Drosophila ovary migratory and non migratory cells in wild type and slbo mutants to identify targets of the critical transcriptional regulator C/EBP homolog SLBO

ABSTRACT: Cell migration contributes to normal development and homeostasis as well as to pathological processes such as inflammation and tumor metastasis. Previous genetic screens have revealed a few major signaling pathways that govern follicle cell migrations in the Drosophila ovary, several of which elicit transcriptional responses. However few downstream targets of the critical transcriptional regulators, such as the C/EBP homolog SLBO, have been identified. To characterize the gene expression profile of two migratory cell populations and identify SLBO targets, we employed a magnetic bead based cell separation approach to purify border cells and centripetal cells expressing the mouse CD8 antigen, and carried out whole genome microarray analysis. Experiment Overall Design: 12 samples were analyzed to compare gene expression between different cell types or gene types. Three biological repeats were done for each experiments.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Xuejiao Wang 

PROVIDER: E-GEOD-4235 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress