Unknown,Transcriptomics,Genomics,Proteomics

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Comprehensive, high resolution analysis of cis-acting regulatory networks at the alpha globin locus (array)


ABSTRACT: We have combined the 4C chromosome conformation capture protocol with high throughput, genome-wide sequence analysis to characterise in full a cis-acting regulatory network at a single locus. In contrast to methods which identify large interacting regions (10-1000kb), the 4C approach provides a comprehensive, high resolution analysis of a specific locus with the aim of defining, in detail, all cis- regulatory elements controlling a single gene or gene cluster. Using the human M-NM-1-globin locus as a model we detected all known local and long-range interactions with this gene cluster. In addition we identified two interactions with genes located 300 kb (NME4) and 625 kb (FAM173a) from the M-NM-1-globin cluster. Using paired-end sequencing it was possible for the first time to identify interactions between specific subsets of cis-regulatory elements in populations of cells and within individual cells. Two biological replicates of 4C amplification from the promoters of the human HBA and HBB genes and B-Cell controls. Quality of B-cell material was confirmed using amplification from a CTCF bound region associated with Human Axin Gene.

ORGANISM(S): Homo sapiens

SUBMITTER: Richard Gibbons 

PROVIDER: E-GEOD-42383 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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High-resolution analysis of cis-acting regulatory networks at the α-globin locus.

Hughes Jim R JR   Lower Karen M KM   Dunham Ian I   Taylor Stephen S   De Gobbi Marco M   Sloane-Stanley Jacqueline A JA   McGowan Simon S   Ragoussis Jiannis J   Vernimmen Douglas D   Gibbons Richard J RJ   Higgs Douglas R DR  

Philosophical transactions of the Royal Society of London. Series B, Biological sciences 20130506 1620


We have combined the circular chromosome conformation capture protocol with high-throughput, genome-wide sequence analysis to characterize the cis-acting regulatory network at a single locus. In contrast to methods which identify large interacting regions (10-1000 kb), the 4C approach provides a comprehensive, high-resolution analysis of a specific locus with the aim of defining, in detail, the cis-regulatory elements controlling a single gene or gene cluster. Using the human α-globin locus as a  ...[more]

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