Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Impaired neutrophil recruitment in CXCL5 knockout mice during Mtb infection

ABSTRACT: Comparison of Mycobacterium tuberculosis infection in WT mice strain C57BL/6 (WTM-BM-^]) and the CXCL5 knockout (KO) Two color arrays with dye swaps, RNA harvested at day 0 (d00, no infection) and day 21 (d21); 5 mice per experimental group. RNA from each experimental group was pooled. The e0, e8, e9 and eX are internal experimental run/labels of equivalent biological replications of the experiments.

ORGANISM(S): Mus musculus

SUBMITTER: January Weiner

PROVIDER: E-GEOD-43337 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

CXCL5-secreting pulmonary epithelial cells drive destructive neutrophilic inflammation in tuberculosis.

Nouailles Geraldine G Dorhoi Anca A Koch Markus M Zerrahn Jens J Weiner January J Faé Kellen C KC Arrey Frida F Kuhlmann Stefanie S Bandermann Silke S Loewe Delia D Mollenkopf Hans-Joachim HJ Vogelzang Alexis A Meyer-Schwesinger Catherine C Mittrücker Hans-Willi HW McEwen Gayle G Kaufmann Stefan H E SH

The Journal of clinical investigation 20140210 3

Successful host defense against numerous pulmonary infections depends on bacterial clearance by polymorphonuclear leukocytes (PMNs); however, excessive PMN accumulation can result in life-threatening lung injury. Local expression of CXC chemokines is critical for PMN recruitment. The impact of chemokine-dependent PMN recruitment during pulmonary Mycobacterium tuberculosis infection is not fully understood. Here, we analyzed expression of genes encoding CXC chemokines in M. tuberculosis-infected ...[more]

PMID: 24509076

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Project description:The mammary gland is a unique apocrine gland made up of a branching network of ducts that end in alveoli. It is an ideal system to study the molecular mechanisms associated with cell proliferation, differentiation, and oncogenesis. MFG-E8, also known as Lactadherin, is a vital glycoprotein related to the milk fat globule membrane and initially identified to get secreted in bovine milk. Our previous report suggests that a high level of MFG-E8 is indicative of high milk yield in dairy animals. Here, we showed that MFGE-E8 controls the cell growth and morphology of epithelial cells through a network of regulatory transcription factors. To understand the comprehensive action, we downregulated its expression in MECs by MFG-E8 specific shRNA. We generated a knockdown proteome profile of differentially expressed proteins through a quantitative iTRAQ experiment on a high-resolution mass spectrometer (Q-TOF). The downregulation of MFG-E8 resulted in reduced phagocytosis and cell migration ability, whereas it also leads to more lifespan to knockdown vis-a-vis healthy cells, which is confirmed through BrdU, MTT, Neutral red, and Caspase 3/7. The bioinformatics analysis revealed that MFG-E8 knockdown perturbs a large number of intracellular signaling, eventually leading to cessation in cell growth. Based on the Weighted Gene Coexpression Network Analysis and directed network, we found that MFG-E8 is activated by CX3CL1, TP63, and CSF2 and leads to the activation of SOCS3 and CCL2 for the regulation of cell proliferation. We further proved that the depletion of MFG-E8 resulted in activated cytoskeletal remodeling by MFG-E8 knockdown, which results in the activation of three independent pathways ZP4/JAK-STAT5, DOCK1/STAT3, and PIP3/AKT/mTOR. Overall, this study suggests that MFG-E8 expression in mammary epithelial cells is an indication of intracellular deterioration in cell health. To date, to the best of our knowledge, this is the first study that explores the downstream targets of MFG-E8 involved in the regulation of mammary epithelial cell health.

Dataset Information

Impaired neutrophil recruitment in CXCL5 knockout mice during Mtb infection

Publications

CXCL5-secreting pulmonary epithelial cells drive destructive neutrophilic inflammation in tuberculosis.

Similar Datasets

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