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Translational landscape of photomorphogenic Arabidopsis

ABSTRACT: We report the application of ribosome profiling method to map ribosome locations on transcripts in de-etiolating Arabidopsis seedlings Examine steady-state mRNA and ribosome-protected mRNA abundance in 4-day-old etiolated seedlings with/without 4-h light treatment

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Ming-Jung Liu

PROVIDER: E-GEOD-43703 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Translational landscape of photomorphogenic Arabidopsis.

Liu Ming-Jung MJ Wu Szu-Hsien SH Wu Jing-Fen JF Lin Wen-Dar WD Wu Yi-Chen YC Tsai Tsung-Ying TY Tsai Huang-Lung HL Wu Shu-Hsing SH

The Plant cell 20131031 10

Translational control plays a vital role in regulating gene expression. To decipher the molecular basis of translational regulation in photomorphogenic Arabidopsis thaliana, we adopted a ribosome profiling method to map the genome-wide positions of translating ribosomes in Arabidopsis etiolated seedlings in the dark and after light exposure. We found that, in Arabidopsis, a translating ribosome protects an ~30-nucleotide region and moves in three-nucleotide periodicity, characteristics also obse ...[more]

PMID: 24179124

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Translational control: a new dimension in the regulation of Arabidopsis photomorphogenesis

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