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The Toxoplasma unfolded protein response

ABSTRACT: To characterize the nature of the unfolded protein response in this Toxoplasma, we carried out microarray analyses to measure the changes in the transcriptome and in translational control during ER stress. Total RNA or RNA fractionated into monosomes or polysomes were purified from parasites treated with DMSO or tunicamycin for 1 hour. RNA was processed for microarray analysis using a ToxoGeneChip.

ORGANISM(S): Toxoplasma gondii

SUBMITTER: Ron Wek

PROVIDER: E-GEOD-43722 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The unfolded protein response in the protozoan parasite Toxoplasma gondii features translational and transcriptional control.

Joyce Bradley R BR Tampaki Zoi Z Kim Kami K Wek Ronald C RC Sullivan William J WJ

Eukaryotic cell 20130510 7

The unfolded protein response (UPR) is an important regulatory network that responds to perturbations in protein homeostasis in the endoplasmic reticulum (ER). In mammalian cells, the UPR features translational and transcriptional mechanisms of gene expression aimed at restoring proteostatic control. A central feature of the UPR is phosphorylation of the α subunit of eukaryotic initiation factor-2 (eIF2) by PERK (EIF2AK3/PEK), which reduces the influx of nascent proteins into the ER by lowering ...[more]

PMID: 23666622

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