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Unknown,Transcriptomics,Genomics,Proteomics

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Sliding of Bacillus subtilis Natto

ABSTRACT: Transcriptome comparison of Bacillus subtilis Natto under sliding permissive (0.7% agar) and restrictive (1.5% agar or spo0A mutant strain) conditions. B subtilis Natto wild type cells were grown on the top of LB solid medium with 1.5% and 0.7% agar concentration (samples 1-4). B subtilis Natto wild type and spo0A derivative were grown on top of LB solid medium with 0.7% agar concentration (Sample 5-7). In first experiment, 4 biological replicates were used, while in the second experiment 3 biological replicates included. Dye swaps are included in both experiments.

ORGANISM(S): Bacillus subtilis subsp. natto

SUBMITTER: Akos Kovacs

PROVIDER: E-GEOD-43840 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A Duo of Potassium-Responsive Histidine Kinases Govern the Multicellular Destiny of Bacillus subtilis.

Grau Roberto R RR de Oña Paula P Kunert Maritta M Leñini Cecilia C Gallegos-Monterrosa Ramses R Mhatre Eisha E Vileta Darío D Donato Verónica V Hölscher Theresa T Boland Wilhelm W Kuipers Oscar P OP Kovács Ákos T ÁT

mBio 20150707 4

<h4>Unlabelled</h4>Multicellular biofilm formation and surface motility are bacterial behaviors considered mutually exclusive. However, the basic decision to move over or stay attached to a surface is poorly understood. Here, we discover that in Bacillus subtilis, the key root biofilm-controlling transcription factor Spo0A~Pi (phosphorylated Spo0A) governs the flagellum-independent mechanism of social sliding motility. A Spo0A-deficient strain was totally unable to slide and colonize plant roots ...[more]

PMID: 26152584

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