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SW1 mutant vs. Shewanella piezotolerans WP3 wild-type strain

ABSTRACT: The prophage and RF DNA of SW1 were removed from WP3 to construct the phage-free strain. The two strains were cultured at 4M-BM-0C and the transcriptional profiles were compared. WP3M-NM-^TSW1 is the test sample and WP3 wild-type is the reference sample. Three independent cultures were used for RNA extraction, and the RNA samples were combined into one sample for microarray. Each microarray slide has 3 spots for a single gene, and as dye-swaps were performed, 6 spots were used to determine the expression level of one gene.

ORGANISM(S): Shewanella piezotolerans WP3

SUBMITTER: Huahua Jian 

PROVIDER: E-GEOD-43882 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Role of filamentous phage SW1 in regulating the lateral flagella of Shewanella piezotolerans strain WP3 at low temperatures.

Jian Huahua H   Xiao Xiang X   Wang Fengping F  

Applied and environmental microbiology 20130913 22

Low-temperature ecosystems represent the largest biosphere on Earth, and yet our understanding of the roles of bacteriophages in these systems is limited. Here, the influence of the cold-active filamentous phage SW1 on the phenotype and gene transcription of its host, Shewanella piezotolerans WP3 (WP3), was investigated by construction of a phage-free strain (WP3ΔSW1), which was compared with the wild-type strain. The expression of 49 genes, including 16 lateral flagellar genes, was found to be  ...[more]

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