Project description:The hns gene was deleted from WP3 genome to construct the WP3M-NM-^Thns strain. The two strains were cultured at 20M-BM-0C and the transcriptional profiles were compared. Three biological replicates sample and 6 technical replicates for each experiment
Project description:The hns gene was deleted from WP3 genome to construct the WP3M-NM-^Thns strain. The two strains were cultured at 4M-BM-0C and the transcriptional profiles were compared. Three biological replicates sample and 6 technical replicates for each experiment
Project description:The prophage and RF DNA of SW1 were removed from WP3 to construct the phage-free strain. The two strains were cultured at 20MPa and 4M-BM-0C and the transcriptional profiles were compared. Three biological replicates sample and 6 technical replicates for each experiment
Project description:The cAMP binding domain of crp gene was deleted from WP3 genome to construct the WP3?crpD1 strain. The two strains were cultured at 20°C and the transcriptional profiles were compared. Three biological replicates sample and 6 technical replicates for each experiment
Project description:The DNA binding domain of crp gene was deleted from WP3 genome to construct the WP3?crpD2 strain. The two strains were cultured at 20°C and the transcriptional profiles were compared. Three biological replicates sample and 6 technical replicates for each experiment
Project description:The prophage and RF DNA of SW1 were removed from WP3 to construct the phage-free strain. The two strains were cultured at 4M-BM-0C and the transcriptional profiles were compared. WP3M-NM-^TSW1 is the test sample and WP3 wild-type is the reference sample. Three independent cultures were used for RNA extraction, and the RNA samples were combined into one sample for microarray. Each microarray slide has 3 spots for a single gene, and as dye-swaps were performed, 6 spots were used to determine the expression level of one gene.
Project description:The WP3 strains was cultured at 20°C and heat shocked at 40°C for 30 min. The transcriptional profiles after and before heat shock were compared. Three biological replicates sample and 6 technical replicates for each experiment
Project description:The WP3 strains was cultured at 20°C and heat shocked at 40°C for 90 min. The transcriptional profiles after and before heat shock were compared. Three biological replicates sample and 6 technical replicates for each experiment
Project description:The WP3 strains was cultured at 20°C and heat shocked at 40°C for 60 min. The transcriptional profiles after and before heat shock were compared. Three biological replicates sample and 6 technical replicates for each experiment