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Transcription profiling of human CD4+ T-cells and GM6990 lymphoblastoid cell lines

ABSTRACT: CD4+ T-cells isolated from three normal individuals and GM6990 cell lines (three biological replicates) are compared Experiment Overall Design: 8 arrays comparing CD4+ T-cells and GM6990 lymphoblastoid cell lines

ORGANISM(S): Homo sapiens

SUBMITTER: Sean Davis 

PROVIDER: E-GEOD-4406 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

DNase-chip: a high-resolution method to identify DNase I hypersensitive sites using tiled microarrays.

Crawford Gregory E GE   Davis Sean S   Scacheri Peter C PC   Renaud Gabriel G   Halawi Mohamad J MJ   Erdos Michael R MR   Green Roland R   Meltzer Paul S PS   Wolfsberg Tyra G TG   Collins Francis S FS  

Nature methods 20060701 7

Mapping DNase I hypersensitive sites is an accurate method of identifying the location of gene regulatory elements, including promoters, enhancers, silencers and locus control regions. Although Southern blots are the traditional method of identifying DNase I hypersensitive sites, the conventional manual method is not readily scalable to studying large chromosomal regions, much less the entire genome. Here we describe DNase-chip, an approach that can rapidly identify DNase I hypersensitive sites  ...[more]

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