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Rickettsia rickettsii transcriptome - Elevation of temperature response

ABSTRACT: One of the most important vectors of the Brazilian Spotted Fever, the tick Amblyomma aureolatum in Brazil was used in this study. We laboratorial controlled the infection of adult females of A. aureolatum with the virulent brazilian strain Taiacu of Rickettsia rickettsii. The group of ticks was divided into 2 testing groups, group 1 (G1) composed of adult females incubated at 25°C for 3 days and group 2 (G2) composed of adult females incubated at 35°C for 3 days. Right after incubation of both groups, ticks were individually dissected and all internal organs were transferred to RNAlater® Solution (Life Technologies) until gDNA and total RNA simultaneously isolation. A total of 14 ticks of each group were analyzed in two biological replicates (7 ticks each). Dye-swap was also applied to construct the technical replicate of each biological sample total RNA from both experimental samples (G1 and G2) was used for hybridization to dual channel arrays. Two biological replicates were used for each experimental group.

ORGANISM(S): Rickettsia rickettsii

SUBMITTER: Maria Galletti

PROVIDER: E-GEOD-44316 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Rickettsia rickettsii transcriptome - Blood feeding response

Project description:One of the most important vectors of the Brazilian Spotted Fever, the tick Amblyomma aureolatum in Brazil was used in this study. We laboratorial controlled the infection of adult females of A. aureolatum with the virulent brazilian strain Taiacu of Rickettsia rickettsii. The group of ticks was divided into 2 testing groups, group 2 (G2) composed of adult females incubated at 35°C for 3 days that were not fed after molting to adults and group 3 (G3) composed of adult females fed on its favorite natural host, the dog (Canis familiaris) also for 3 days. Right after incubation or feeding, ticks were individually dissected and all internal organs were transferred to RNAlater® Solution (Life Technologies) until gDNA and total RNA simultaneously isolation. A total of 14 ticks of each group were analyzed in two biological replicates (7 ticks each). Dye-swap was also applied to construct the technical replicate of each biological sample total RNA from both experimental samples (G2 and G3) was used for hybridization to dual channel arrays. Two biological replicates were used for each experimental group.

2013-12-01 | E-GEOD-44315 | biostudies-arrayexpress

Natural blood feeding and temperature shift modulate the global transcriptional profile of Rickettsia rickettsii.

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-12-01 | E-GEOD-44349 | biostudies-arrayexpress

Circadian rhythms of sense and antisense transcription in a highly polyploid crop.

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2013-11-21 | E-GEOD-42725 | biostudies-arrayexpress

Sugarcane Expression Data from Stress Time Series

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Transcription profiling of human treated cord blood-derived human mast cells control and TGF-beta treated

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2008-06-20 | E-GEOD-10291 | biostudies-arrayexpress

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Microarray analysis of gene expression differences in prostate specific antigen negative (PSA-ve) cells versus PSA+ve cells in LAPC9 and LNCaP prostate cancer (PCa) cells

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The PSA-/lo prostate cancer cells harbor self-renewing long-term tumor-propagating cells that resist castration

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2012-05-09 | E-GEOD-35814 | biostudies-arrayexpress

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