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HMLER cells expressing either FOXC2 or a vector control

ABSTRACT: We used microarrays to investigate the transcription profile of FOXC2 expression in a human mammary epithelial cell line. HMLER cells were infected with either a control vector or a retroviral vector expressing FOXC2.

ORGANISM(S): Homo sapiens

SUBMITTER: Jeffrey Chang

PROVIDER: E-GEOD-44335 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression analysis of colorectal cancer cell line SW480 expressing FOXC2

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HMLER cells expressing either FOXC2 or a vector control

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Transcription profiling of white adipose tissue of transgenic mice overexpressing the FOXC2 gene compared with adipose tissue of wild type mice

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2007-04-25 | E-MEXP-410 | biostudies-arrayexpress

ChIP-chip study aimed at the identification of the repertoire of FOXC2 binding sites affected by the loss of FOXC2 phosphorylation in primary human lymphatic endothelial cells (LECs)

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Transcription profiling of human breast tumor epithelia cell types HMLER and BPLER compared to immortalized parent cells of origin - HME and BPE

Project description:The gene expression of two different tumorigenic human breast epithelial cell types (HMLER and BPLER) is compared with their immortalized parental cell-of-origin (HME and BPE). Experiment Overall Design: Two different normal primary human mammary epithelial cell populations (BPECs and HMECs) were isolated based on their differing in vitro growth requirements. These cells were immortalized by hTERT giving rise to BPE and HME cells. These hTERT immortalized cells (BPE and HME) were transformed by SV40-early region (LT+st) and H-Ras giving rise to transformed tumorigenic derivatives BPLER and HMLER. Biological replicates (4 - 6 samples) for each of 4 cell types were analyzed (untransformed hTERT immortalized cell populations (BPE&HME), and transformed tumorigenic derivatives (BPLER & HMLER).

2008-06-15 | E-GEOD-6885 | biostudies-arrayexpress

Expression profile of human lymphatic endothelial cells under static or oscillatory shear stress conditions in the presence or absence of FOXC2

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Transcription profiling of human HMLER breast cancer cells treated with paclitaxel and salinomycin-treated HMLER breast cancer cells

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2009-09-15 | E-GEOD-17215 | biostudies-arrayexpress

Homo sapiens

Project description:HMLER cells expressing either FOXC2 or a vector control

| PRJNA189480 | ENA

Genome-wide analysis of the RNA polyemerase I trascription factor UBF1/2 binding in mouse and human cell lines.

Project description:We report ChIP-Seq analysis of the RNA polyemerase I trascription factor UBF1/2 in NIH3T3, HMEC and HMLER cell lines. NIH3T3* samples: We correlated UBF1/2 binding across the genome with that of RNA polemerase I (Pol I), RNA polemerase II (Pol II) and chromatin states in NIH3T3 cells We perfromed ChIP-seq of UBF1/2 (2 biological replicates), Pol I (POLR1A/RPA194), Pol II, H3K9me3, H3K4Me3, H3K9ac, H4 hyperacetylation and genomic DNA input as reference. HMEC* samples: We report ChIP-Seq analysis of UBF1/2 and Pol I (POLR1A/RPA194) in human mammary epithelial cells (HMEC). In addition we include UBF1/2 ChIP-seq in the tumourigenic epithelial cell line HMLER. In this study, we compared UBF1/2 binding in HMEC to UBF1/2 binding in in the tumorigenic HMLER cells, an isogenic HMEC-derived cell line expressing the SV40 large-T, TERT, and an oncogenic allele of the HRAS gene (expressing HRASV12G) that forms tumors in nude mice (Elenbaas et al., Gene & Development 2001) We sequenced UBF1/2 ChIP and Pol I(POLR1A/RPA194) ChIP and gDNA input from HMEC. We sequenced UBF1/2 ChIP and gDNA input from HMLER

2014-11-14 | E-GEOD-63255 | biostudies-arrayexpress

Single Cell RNA-seq data of parental HMLER cells and HMLER-derived clones C1 and C2

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2022-06-27 | GSE206855 | GEO

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