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Gene expression in mouse leukocytes after CAWS administration

ABSTRACT: We performed DNA microarray analyses and compared the difference in the transcriptome between B6 and DBA/2 mice with modest or dramatic sensitivity to CAWS-vasculitis, respectively. Candida albicans water-soluble fraction (CAWS), a mannoprotein-M-NM-2-glucan complex obtained from the culture supernatant of C. albicans NBRC1385, causes CAWS mediated vasculitis (CAWS-vasculitis) with distinct sensitivity among mouse strains. The mouse leukocyte derived from B6 and DBA/2 mice after 2-, 4-, 8-, 9- weeks administration were examined. Each mice was run in singlete or duplicate. Microarray analyses were performed as single-color hybridizations.

ORGANISM(S): Mus musculus

SUBMITTER: Daisuke Okuzaki

PROVIDER: E-GEOD-44803 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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CAWS administration increases the expression of interferon γ and complement factors that lead to severe vasculitis in DBA/2 mice.

Nagi-Miura Noriko N Okuzaki Daisuke D Torigata Kosuke K Sakurai Minami A MA Ito Akihiko A Ohno Naohito N Nojima Hiroshi H

BMC immunology 20130924

<h4>Background</h4>Candida albicans water-soluble fraction (CAWS), a mannoprotein-β-glucan complex obtained from the culture supernatant of C. albicans NBRC1385, causes CAWS-mediated vasculitis (CAWS-vasculitis) in B6 and DBA/2 mice with mild and lethal symptoms, respectively. Why CAWS is lethal only in DBA/2 mice remains unknown.<h4>Results</h4>We performed DNA microarray analyses using mRNA obtained from peripheral blood mononuclear cells (PBMCs) of B6 and DBA/2 mice and compared their respect ...[more]

PMID: 24063402

Similar Datasets

Gene expression in mouse leukocytes after CAWS administration

Project description:We performed DNA microarray analyses and compared the difference in the transcriptome between B6 and DBA/2 mice with modest or dramatic sensitivity to CAWS-vasculitis, respectively. Candida albicans water-soluble fraction (CAWS), a mannoprotein-β-glucan complex obtained from the culture supernatant of C. albicans NBRC1385, causes CAWS mediated vasculitis (CAWS-vasculitis) with distinct sensitivity among mouse strains.

2013-09-30 | GSE44803 | GEO

Genopal: a novel platform for focused microarray analysis

Project description:This SuperSeries is composed of the following subset Series: GSE16945: Comparative transcription profiling of peripheral blood mononuclear cells from vasculitis patients GSE18247: Focused transcription profiling of peripheral blood mononuclear cells from vasculitis patients Refer to individual Series

2010-11-10 | E-GEOD-18316 | biostudies-arrayexpress

Comparative transcription profiling of peripheral blood mononuclear cells from vasculitis patients

Project description:Peripheral blood mononuclear cells (PBMCs) from 13 vasculitis patients expressed varying gene profiles. A total of 13 sets of two color arrays were performed and each individual array included a target sample and a control sample.

2010-11-10 | E-GEOD-16945 | biostudies-arrayexpress

Focused transcription profiling of peripheral blood mononuclear cells from vasculitis patients

Project description:Peripheral blood mononuclear cells (PBMCs) from 13 vasculitis patients expressed varying gene profiles. A total of 14 sets of one color arrays were performed. Four technical replicates were performed for each sample.

2010-11-10 | E-GEOD-18247 | biostudies-arrayexpress

Enhanced expression of Ficolin 1 is a specific gene marker of Takayasu's vasculitis

Project description:Takayasu's arteritis (TA) Vasculitis (angiitis) is a chronic inflammatory disease involving large blood vessels. We here identify the genes whose mRNA levels are commonly augmented in the peripheral blood mononuclear cells (PBMCs) from TA patients regardless of the patient's symptom, active or inactive phase, compared with those of normal volunteers. Among them, ficolin 1 (FCN1) was notable because its mRNA level was more than 4.0 fold in all 8 patients examined. A total of 8 two color arrays were performed and each individual array included a target sample and a control sample.

2012-03-26 | E-GEOD-33910 | biostudies-arrayexpress

Nucleoredoxin is required for the maintenance of Wnt/Î²-catenin signaling in mice embryo

Project description:We previously showed that nucleoredoxin (NRX) suppresses Wnt/Î²-catenin signaling through its binding to Dishevelled (Dvl) (Nat Cell Biol 8, 501-508 (2006)). To clarify the in vivo role of NRX in mammals, we here generate NRX gene-knockout mice (NRX-/- mice) by homologous recombination. NRX-/- mice die around birth. Therefore, we performed microarray analyses with NRX+/+ and NRX-/- embryos of E9.5 and E11.5 stages. Surprisingly, in the genes commonly upregulated at both stages, we could not observe Wnt/Î²-catenin targets. Rather, several target genes for Wnt/Î²-catenin pathway, such as Frizzled2 and Occludin, are downregulated in NRX-/- whole embryos. Frizzled2 is a gene reportedly expressed in developmental heart. Indeed, by RT-PCR analyses we confirmed that the expression of Frizzled2, as well as other Wnt/Î²-catenin target genes, was downregulated in embryonic heart of NRX-/- mice. We also found that the amount of unphosphorylated (i.e. activated) form of Î²-catenin was downregulated in NRX-/- embryonic heart. These results reveal that NRX plays another role which was unidentified in culture cell studies; it is required for the maintenance of Wnt/Î²-catenin signaling activity. Total RNAs were extracted from E9.5 and E11.5 embryos derived from NRX+/+ and NRX-/- C57BL/6J mice using RNeasy extraction kit (Qiagen). Two dye-swapped experiments were performed by hybridizing complimentary RNA (cRNA) labeled with either Cyanine (Cy) -3 or Cy-5 (Perkin-Elmer) onto Whole Mouse Genome Oligo Microarray (G4122A; Agilent Technologies). The signature genes with mean fold changes > +2.0 or < -2.0 at both stages were subjected for further evaluation.

2010-10-26 | E-GEOD-21954 | biostudies-arrayexpress

Expression profiling in primary embryonic fibroblasts deficient for the N-terminal truncation of Lats1

Project description:Lats1 and Lats2 are the mediators of the Hippo pathway that regulates tissue growth and proliferation. Lats1 and Lats2 kinases share 85% sequence identity in the kinase domain. However, their non-kinase regions at the N-terminus are distinct except for Lats conserved domain 1 (LCD1) and LCD2, suggesting that their N-terminal regions are important for Lats1/2-specific functions. In this study, we generated Lats1 knockout mice disrupting the N-terminal region containing LCD1 (Lats1M-NM-^TN/M-NM-^TN). We show that some Lats1M-NM-^TN/M-NM-^TN mice were born safely and grew normally. However, mouse embryonic fibroblasts (MEFs) from Lats1M-NM-^TN/M-NM-^TN mice displayed drastic defects in mitosis, showing enhanced centrosome overduplication, chromosomal misalignment, multipolar spindle formation, chromosome bridging, and cytokinesis failure. Moreover, they displayed accelerated cell cycle and cell growth bypassing a cell-cell contact inhibition like tumor cells, and exhibited anchorage independent growth. Indeed, Lats1M-NM-^TN/M-NM-^TN MEFs produced tumors in nude mice after subcutaneous injection, although the tumor growth rate was much slower than ordinary cancer cells. Furthermore, Yap, the key transcriptional co-activator in the Hippo pathway, was overexpressed and retained stable in Lats1M-NM-^TN/M-NM-^TN MEFs under high cell density, and expression of Lats2 mRNA were down-regulated. Total RNAs were extracted from MEFs under high or low cell density using miRNeasy extraction kit (Qiagen). A Dye-swapped experiment was performed by hybridizing complimentary RNA (cRNA) labeled with either Cyanine (Cy) -3 or Cy-5 (Perkin-Elmer) onto Whole Mouse Genome Oligo Microarray (G4122F; Agilent Technologies). Co-hybridizations were performed and data from Cy3 or Cy5 channels were analyzed as normalized signal intensities rather than as log ratios corresponding to each array.

2012-12-17 | E-GEOD-37871 | biostudies-arrayexpress

Genome wide DNA methylation profiling of whole blood samples from C57BL/6 and DBA/2J healthy mice

Project description:Aging of mice can be tracked by DNA methylation changes at specific sites in the genome. In this study, we used the Infinium Mouse Methylation BeadChip to compare such epigenetic modifications in C57BL/6 (B6) and DBA/2J (DBA) mice. There were marked differences in age-associated DNA methylation in these commonly used mouse strains. In B6 the age-associated DNA methylation revealed general age-associated hypomethylation with focused hypermethylation at CpG islands, whereas this was hardly observed in DBA mice. The CpGs with highest age-correlation were overlapping in B6 and DBA and included the genes Hsf4, Prima1, Aspa, and Wnt3a. Notably, Hsf4, Prima1 were also top candidates in previous studies based on whole genome deep sequencing approaches. Furthermore, Hsf4, Aspa, and Wnt3a revealed highly significant age-associated DNA methylation in the homologous regions in human. Taken together, age-associated DNA methylation differs between B6 and DBA, but the most prominent regions are conserved, even in humans.

2022-06-21 | GSE200527 | GEO

Gene expression in the adrenal glands of two spontaneously hypertensive rat strains at 3 and 6 weeks of age

Project description:We examined gene expression profiles in the rat adrenal glands using genome-wide microarray technology, and determined gene expression profiles in 3 rat strains: normotensive WKY, spontaneously hypertensive rats (SHR), and stroke-prone SHR (SHRSP). To identify candidate genes involved in the genesis of hypertension in the SHR strains, we compared the gene expression levels at 3 and 6 weeks of age, isolated 407 genes showing a more than 4-fold increase or a less than 1/4-fold decrease. The rat adrenal glands derived from normotensive WKY, spontaneously hypertensive rats (SHR), and stroke-prone SHR (SHRSP) wereM-cM-^@M-^@examined. Each strain was run in triplicate.

2013-04-16 | E-GEOD-31457 | biostudies-arrayexpress

Gene expression in the whole brains of two spontaneously hypertensive rat strains at 3 and 6 weeks of age

Project description:We examined gene expression profiles in the rat whole brains using genome-wide microarray technology, and determined gene expression profiles in 3 rat strains: normotensive WKY, spontaneously hypertensive rats (SHR), and stroke-prone SHR (SHRSP). To identify candidate genes involved in the genesis of hypertension in the SHR strains, we compared the gene expression levels at 3 and 6 weeks of age, isolated 407 genes showing a more than 4-fold increase or a less than 1/4-fold decrease. The rat whole brains derived from normotensive WKY, spontaneously hypertensive rats (SHR), and stroke-prone SHR (SHRSP) wereã??examined. Each strain was run in triplicate.

2014-01-24 | E-GEOD-41452 | biostudies-arrayexpress

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