Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of mouse primary FDC and BMDC

ABSTRACT: expression profiles of FDC and BMDC are compared Experiment Overall Design: expression profiles of FDC and BMDC are compared

ORGANISM(S): Mus musculus

SUBMITTER: Masaki Hikida

PROVIDER: E-GEOD-4535 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Establishment of lymphotoxin beta receptor signaling-dependent cell lines with follicular dendritic cell phenotypes from mouse lymph nodes.

Nishikawa Yumiko Y Hikida Masaki M Magari Masaki M Kanayama Naoki N Mori Masaharu M Kitamura Hiroshi H Kurosaki Tomohiro T Ohmori Hitoshi H

Journal of immunology (Baltimore, Md. : 1950) 20061001 8

Follicular dendritic cells (FDCs) have been shown to play a crucial role in the positive selection of high-affinity B cells that are generated by somatic hypermutation in germinal center (GC). Because of technical difficulties in preparing and maintaining pure FDCs, a role for FDCs in this complicated process has not been fully elucidated. In this study, we established a cell line designated as pFL that retained major FDC phenotypes from a three-dimensional culture of mouse lymph node cells. pFL ...[more]

PMID: 17015706

Similar Datasets

primary FDC

Project description:expression profiles of FDC and BMDC are compared Keywords: cell type comparison

2006-10-04 | GSE4535 | GEO

mRNA expression profile in wild-type and Lba1 (Trank1) KO bone marrow derived dendritic cell (BMDC) infected with Sendai virus

Project description:mRNA expression profiles were compared between wild-type and Lba1 KO BMDC infected with Sendai virus.

2024-02-15 | GSE225326 | GEO

Expression data from BMDC cells with or without Sendai virus infection

Project description:Innate immunity is the first line of defense against viral and microbial pathogens. BMDC is critical for innate immunity. To investigate the complicated net signaling after virus invasion, we did a cDNA microarray analysis of BMDC with or without Sendai Virus infection. We used microarrays to find proteins that upregulated by Sendai Virus infection and investigated if these proteins had functions in regulating Sendai Virus induced signaling pathway. BMDC cells are seperated from C57BL6 mice, infected with Sendai Virus or not,cultured and harveseted for RNA extraction and hybridization on Affymetrix microarrays

2011-12-08 | E-GEOD-32499 | biostudies-arrayexpress

Comparison of immature BMDC to BMDC after nkt cell modulation

Project description:Analyze the gene expression profile of BMDC before and after nkt cell modulation.

2009-10-17 | GSE18547 | GEO

Expression data from BMDC

Project description:Expression data from BMDC

| PRJNA435415 | ENA

Comparison of immature BMDC to BMDC after nkt cell modulation

Project description:Analyze the gene expression profile of BMDC before and after nkt cell modulation. Total RNA was extracted from CD11c cells with TRIzol Reagent, purified with RNeasy Kit (Qiagen) and subsequently amplificated using the one cycle target labeling kit according to the AffymetrixGeneChipÂ® Expression Analysis Technical Manual (Affimetrix). Fragmented cRNAs were probed to AffymetrixGeneChipÂ® Mouse Genome 430 2.0 arrays for 16 hours at 45Â°C in the AffymetrixHybridization Oven 640. The arrays were then washed and stained with streptavidin-phycoerythrin using the AffymetrixFluidic Station 450/250 and scanned using the AffymetrixGeneChipÂ® Scanner 3000. The images were analyzed using GCOCÂ® Software and comparison analyses carried out according to the instructions provided by Affymetrix. Each experiment was performed in triplicates and repeated two times. Data handling was mainly done using Bioconductor Software. The probe set-based summary data were log transformed and normalized for probe set intensity-dependent biases. The identification of differentially expressed genes was addressed using linear modeling approach and empirical Bayer methods together with false discovery rate correction of the p-value. We considered a gene to be differentially increased in nkt-DCs only if its expression intensity was significantly changed (p<0.001) compared to Untr-DCs.

2010-03-03 | E-GEOD-18547 | biostudies-arrayexpress

Gene expression dynamics of Flt3L induced ex vivo BMDC differentiation

Project description:To understand the gene expression dynamics during Flt3L induced Bone-marrow derived dendritic cell (BMDC) differentatiion we performed RNA-seq. Three distinct stages were chosen and included the initial bone marrow cells, the Common Myeloid Progenitor (CMP), and fully differentiated BMDC.

2023-11-06 | GSE107026 | GEO

Transcription profiling of mouse bone marrow-derived dendritic cells (BMDC), with cells that had been cocultured (1:5, tumor:BMDC) with mouse ovarian surface epithelial cell line (1D8) cells, with irradiated (50Gy) 1D8 cells, or with the supernatent from 1D8 cells (25%, v/v)

Project description:The Suppressor of cytokine signaling (SOCS) family of negative regulatory proteins are upregulated in response to several cytokines and pathogen-associated molecular patterns (PAMPs), and suppress cellular signaling responses by binding receptor phosphotyrosine residues. Exposure of bone marrow-derived dendritic cells (BMDCs) to 1D8 cells, a murine model of ovarian carcinoma, suppresses their ability to express CD40 and stimulate antigen specific responses in response to PAMPs, and in particular to poly I: C with the upregulated SOCS3 transcript and protein levels. The ectopic expression of SOCS3 in both the macrophage cell line RAW264.7 and BMDCs decreased signaling in response to both poly I:C and IFNÎ±. Further, knockdown of SOCS3 transcripts significantly enhanced the responses of RAW264.7 and BMDCs to both poly I: C and IFNÎ±. Immunoprecipitation and pull-down studies demonstrate that SOCS3 binds to the IFNÎ± receptor TYK2. Since poly I: C triggers autocrine IFNÎ± signaling, binding of SOCS3 to TYK2 may thereby suppress the activation of BMDCs by polyI:C and IFNÎ±. Thus, elevated levels of SOCS3 in tumor-associated DCs may potentially resist the signals induced by TLR3 ligands and type I interferon to decrease DC activation via binding with IFNÎ± receptor TyK2. Experiment Overall Design: Microarray analysis was used to compare the expression levels of Control mouse bone marrow-derived dendritic cells (BMDC), with cells that had been cocultured (1:5, tumor:BMDC) with mouse ovarian surface epithelial cell line (1D8) cells, with irradiated (50Gy) 1D8 cells, or with the supernatent from 1D8 cells (25%, v/v). Experiment Overall Design: One biologic sample was analyzed for each condition, four samples in all.

2009-02-28 | E-GEOD-10741 | biostudies-arrayexpress

DNA methylation dynamics of Flt3L induced ex vivo BMDC differentiation

Project description:To understand the DNA methylation dynamics during Flt3L induced Bone-marrow derived dendritic cell (BMDC) differentatiion we performed reduced representation bisulfite sequencing (RRBS). Three distinct stages were chosen and included the initial bone marrow cells, the Common Myeloid Progenitor (CMP), and fully differentiated BMDC.

2023-11-06 | GSE107027 | GEO

Gene expressions of BMDC with or without Rbpj

Project description:cDNA microarray data of Bone marrow-derived dendritic cells (BMDC) with or without Rbpj gene

2017-11-27 | GSE100593 | GEO