Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Mitochondrial two-component signaling systems in Candida albicans

ABSTRACT: Two-component signal transduction pathways are one of the primary means by which microorganisms respond to environmental signals. These signaling cascades originated in prokaryotes and were inherited by eukaryotes via endosymbiotic lateral gene transfer from ancestral cyanobacteria. We report here that the nuclear genome of pathogenic fungus Candida albicans contains elements of a two-component signaling pathway that seem to be targeted to the mitochondria. In C. albicans two-component response regulator protein Srr1(Stress Response Regulator) contains a mitochondrial targeting sequence at the N-terminus, and fluorescence microscopy reveals mitochondrial localization of GFP-tagged Srr1p. Moreover, phylogenetic analysis indicates that C. albicans Srr1p is more closely related to histidine kinases and response regulators found in marine bacteria compared to other two-component proteins present in the fungi. These data suggest conservation of this protein during the evolutionary transition from endosymbiont to a subcellular organelle. We used microarray analysis to determine if the phenotypes observed with srr1M-NM-^T/M-NM-^T mutant could be correlated with gene transcriptional changes. Expression of mitochondrial genes was altered in the srr1M-NM-^T/M-NM-^T null mutant in comparison to the wild type. Furthermore, apoptosis significantly increased in the srr1M-NM-^T/M-NM-^T mutant strain compared to wild type, suggesting activation of mitochondria dependent apoptotic cell death pathway in the srr1M-NM-^T/M-NM-^T mutant. This study shows for the first time that a lower eukaryote like C. albicans possesses a two-component response regulator protein that has survived in mitochondria and regulates a subset of genes whose functions are associated with oxidative stress response and programmed cell death (apoptosis). Candida albicans wildtype or srr1 null cells were left untreated or were treated with either 8mM H2O2 for one hour prior to RNA isolation.

ORGANISM(S): Candida albicans

SUBMITTER: Kathy Barker

PROVIDER: E-GEOD-45438 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Mitochondrial two-component signaling systems in Candida albicans.

Mavrianos John J Berkow Elizabeth L EL Desai Chirayu C Pandey Alok A Batish Mona M Rabadi Marissa J MJ Barker Katherine S KS Pain Debkumar D Rogers P David PD Eugenin Eliseo A EA Chauhan Neeraj N

Eukaryotic cell 20130412 6

Two-component signal transduction pathways are one of the primary means by which microorganisms respond to environmental signals. These signaling cascades originated in prokaryotes and were inherited by eukaryotes via endosymbiotic lateral gene transfer from ancestral cyanobacteria. We report here that the nuclear genome of the pathogenic fungus Candida albicans contains elements of a two-component signaling pathway that seem to be targeted to the mitochondria. The C. albicans two-component resp ...[more]

PMID: 23584995

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Dataset Information

Mitochondrial two-component signaling systems in Candida albicans

Publications

Mitochondrial two-component signaling systems in Candida albicans.

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