Unknown,Transcriptomics,Genomics,Proteomics

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Thermotoga maritima transcription during exponential and stationary phase growth on 1 and 5 g/l yeast extract

ABSTRACT: Although proteins and peptides encoded in small open reading frames (ORFs < 100 AA) in microbial genomes can play critical roles as toxins, bacteriocins, transcriptional regulators, signaling molecules, and chaperones, many such ORFs remain annotated as M-bM-^@M-^\hypothetical proteinsM-bM-^@M-^]. In the genome of the hyperthermophilic bacterium, Thermotoga maritima, nearly 2/3 of the 167 small ORFs have no known function. As a strategy for investigating the potential significance of specific small ORFs, growth conditions that could trigger the expression of genes encoding small ORFs were sought. A defined medium supplemented with either 1 or 5 g/L yeast extract was used to track transcriptional response during the transition from exponential to stationary phase. RNA derived from exponential (1E) and stationary (1S) phase cultures grown on 1 g/l yeast extract was compared to RNA derived from exponential (5E) and stationary (5S) phase cultures grown on 5 g/l yeast extract using a four-slide loop design.

ORGANISM(S): Thermotoga maritima

SUBMITTER: Andrew Frock

PROVIDER: E-GEOD-47072 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Thermotoga maritima transcription during exponential and stationary phase growth on 1 and 5 g/l yeast extract

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