Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Off-target effects of VEGF-A regulation by shRNAs


ABSTRACT: The aim of this project was to investigate how genome-wide gene expression patterns change when the expression of VEGF-A is modulated using different lentivirally delivered shRNA molecules that are complementary to VEGF-A promoter region. To study these changes, 12 gene expression array experiments were conducted with mouse endothelial cell line (C166). The C166 cells were transduced (20% confluency, MOI 10) with lentiviral vectors that all express GFP marker gene and two of them express additional shRNA molecule that is complementary to different regions in the VEGF-A promoter and either up-regulate (VEGF-up) or downregulate (VEGF-down) VEGF-A expression. Fresh medium was changed 72h after transduction. Cells were collected at day 7.

ORGANISM(S): Mus musculus

SUBMITTER: Mikko Turunen 

PROVIDER: E-GEOD-47444 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2015-08-06 | GSE47444 | GEO
2012-10-13 | E-GEOD-41567 | biostudies-arrayexpress
2014-01-16 | E-GEOD-52363 | biostudies-arrayexpress
2013-12-01 | GSE48880 | GEO
2013-12-10 | E-GEOD-53140 | biostudies-arrayexpress
2013-12-01 | E-GEOD-48880 | biostudies-arrayexpress
2020-12-24 | GSE92893 | GEO
2011-12-06 | GSE32385 | GEO
2021-02-01 | GSE165247 | GEO
2022-10-04 | GSE132953 | GEO