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Genomic responses in blood during acute human anaphylaxis

ABSTRACT: Gene expression was profiled in peripheral blood samples collected over three time points from patients during acute anaphylaxis and from healthy controls. Patients presented to the Emergency Department (ED) at Royal Perth Hospital with acute, moderately severe anaphylaxis. Samples were collected at ED arrival (T0), 1 hour later (T1), and 3 hours post arrival (T2). The study design consisted of 12 subjects, 3 time points, and 2 clinical states (acute anaphylaxis, healthy controls).

ORGANISM(S): Homo sapiens

SUBMITTER: Anthony Bosco

PROVIDER: E-GEOD-47655 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genomic responses during acute human anaphylaxis are characterized by upregulation of innate inflammatory gene networks.

Stone Shelley F SF Bosco Anthony A Jones Anya A Cotterell Claire L CL van Eeden Pauline E PE Arendts Glenn G Fatovich Daniel M DM Brown Simon G A SG

PloS one 20140701 7

<h4>Background</h4>Systemic spread of immune activation and mediator release is required for the development of anaphylaxis in humans. We hypothesized that peripheral blood leukocyte (PBL) activation plays a key role.<h4>Objective</h4>To characterize PBL genomic responses during acute anaphylaxis.<h4>Methods</h4>PBL samples were collected at three timepoints from six patients presenting to the Emergency Department (ED) with acute anaphylaxis and six healthy controls. Gene expression patterns wer ...[more]

PMID: 24983946

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