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Gene expression patterns in house dust mite stimulated CD4 T cells and IgG:IgE ratios

ABSTRACT: PBMC from house dust mite (HDM) sensitized atopics were cultured in the presence or absence of HDM extract for 24 hours. At the termination of the cultures, CD4 T cells were isolated using immunomagnetic separation. Gene expression was profiled on microarrays. The study design consisted of 45 subjects and two conditions (medium control, HDM stimulation).

ORGANISM(S): Homo sapiens

SUBMITTER: Anthony Bosco

PROVIDER: E-GEOD-70760 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Distinguishing benign from pathologic TH2 immunity in atopic children.

Holt Patrick G PG Strickland Deborah D Bosco Anthony A Belgrave Danielle D Hales Belinda B Simpson Angela A Hollams Elysia E Holt Barbara B Kusel Merci M Ahlstedt Staffan S Sly Peter D PD Custovic Adnan A

The Journal of allergy and clinical immunology 20151027 2

<h4>Background</h4>Although most children with asthma and rhinitis are sensitized to aeroallergens, only a minority of sensitized children are symptomatic, implying the underlying operation of efficient anti-inflammatory control mechanisms.<h4>Objective</h4>We sought to identify endogenous control mechanisms that attenuate expression of IgE-associated responsiveness to aeroallergens in sensitized children.<h4>Methods</h4>In 3 independent population samples we analyzed relationships between aeroa ...[more]

PMID: 26518094

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Gene expression patterns in allergen-driven CD4 T cell responses from human atopics with or without asthma.

Project description:PBMC from house dust mite (HDM) sensitized atopics with or without asthma (or nonallergic controls) were cultured in the presence or absence of HDM extract for 24 hours. At the termination of the cultures, immunomagnetic separation was performed to purify CD4 T cells. Gene expression was profiled on microarrays. The study design consisted of 72 subjects, and two experimental conditions (medium control, HDM stimulation).

2016-02-25 | E-GEOD-73482 | biostudies-arrayexpress

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2015-07-01 | E-GEOD-46861 | biostudies-arrayexpress

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Project description:Purpose: We have used microarrays to identify gene expression profiles that distinguish mouse OS cells from normal pre-osteoblast cells and mature osteoblast cells. Methods: Transcriptional profiles of three cell lines derived from tumors from Osx-Cre p53fl/fl Rbfl/fl (fibroblastic OS) mouse model, and from pre-osteoblast cells (Kusa4b10 mouse bone marrow stromal cell line) and osteoblast cells (derived by in vitro differentiation of the Kusab410 mouse bone marrow stromal cell line) were generated by microarray analysis, each in triplicate, using Affymetrix mouse Gene1.0ST arrays. Transcriptional profiles were analyzed in cell lines derived from tumors from a genetically engineered mouse model of human osteosarcoma (Osx-Cre p53fl/fl Rbfl/fl) and osteoblast cells derived from the Kusa4b10 mouse bone marrow stromal cell line, in the undifferentiated state (pre-osteoblasts) and differentiated state (osteoblasts).

2015-01-29 | E-GEOD-63939 | biostudies-arrayexpress

Wnt inhibitory factor 1 (WIF1) is not silenced by DNA methylation in osteosarcoma, and instead, represents a marker of osteoblastic differentiation stage (gene expression)

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2015-02-25 | E-GEOD-48219 | biostudies-arrayexpress

Overexpression of cSki in hematopoietic stem and progenitor cells

Project description:Human cSki was overexpressed using MIGR1 retrovirus in sorted murine Lin-c-Kit+Sca-1+ cells. Cells were infected and cultured for 2 days after infection prior to isolation of GFP+ve cells and microarray. GFP+ve MIGR1 and cSKI cells were compared. Each sample represents an independent infection with either cSki or MIGR1 Comparison of GFP+ve LKS+ infected with MIGR1 and cSki

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