Transcriptional analysis of select CfMNPV genes and some antisense transcripts by an oligonucleotide microarray
Ontology highlight
ABSTRACT:
ORGANISM(S): Choristoneura fumiferana MNPV
SUBMITTER: Mehrnoosh Barari
PROVIDER: E-GEOD-4783 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Development of an oligonucleotide-based DNA microarray for transcriptional analysis of Choristoneura fumiferana nucleopolyhedrovirus (CfMNPV) genes.
Yang Dan-Hui DH Barari Mehrnoosh M Arif Basil M BM Krell Peter J PJ
Journal of virological methods 20070410 2
A modified oligonucleotide-based two-channel DNA microarray was developed for characterization of temporal expression profiles of select Choristoneura fumiferana nucleopolyhedrovirus (CfMNPV) ORFs including its 7 unique ORFs. The microarray chip contained oligonucleotide probes for 23 CfMNPV ORFs and their complements as well as five host genes. Total RNA was isolated at different times post infection from Cf203 insect cells infected with CfMNPV. The cDNA was synthesized, fluorescent labelled wi ...[more]
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