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The gene expression changes in darkcd4 apoptosis-deficient mutant flies

ABSTRACT: Apoptosis is required not only for proper morphogenesis and tissue size regulation during development, but also for removing damaged or unwanted cells to maintain homeostasis. Apoptosis is mainly executed by evolutionally-conserved signaling process including caspases and its activator, dark. We performed microarray analysis of darkcd4 mutant flies to reveal the changes in gene expression. We found the elevated immune reaction, suggesting that inflammatory response was triggered by necrosis in darkcd4 . We collected total RNA from day 5 adult of darkcd4 mutant flies backcrossed six generations to w1118. w1118 was used as control. N=4 for each sample.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Masayuki Miura

PROVIDER: E-GEOD-47853 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Necrosis-driven systemic immune response alters SAM metabolism through the FOXO-GNMT axis.

Obata Fumiaki F Kuranaga Erina E Tomioka Katsura K Ming Ming M Takeishi Asuka A Chen Chun-Hong CH Soga Tomoyoshi T Miura Masayuki M

Cell reports 20140417 3

Sterile inflammation triggered by endogenous factors is thought to contribute to the pathogenesis of acute and chronic inflammatory diseases. Here, we demonstrate that apoptosis-deficient mutants spontaneously develop a necrosis-driven systemic immune response in Drosophila and provide an in vivo model for studying the organismal response to sterile inflammation. Metabolomic analysis of hemolymph from apoptosis-deficient mutants revealed increased sarcosine and reduced S-adenosyl-methionine (SAM ...[more]

PMID: 24746817

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