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RNA-Seq on Illumina platform of Drosophila DrosDel deficiency lines

ABSTRACT: RNA-Seq reads of DrosDel deficiency (Df/+) and parental strain diploid flies along with spike-in controls was performed on the Illumina platform. Samples are named in this dataset according to the following sample naming scheme: tissue_genotype shorthand_sex_biological replicate #_platform. We sequenced mRNA from pools of female or male DrosDel and w1118 (parental strain for the Df/+ flies).

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Brian Oliver

PROVIDER: E-GEOD-31549 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Mediation of Drosophila autosomal dosage effects and compensation by network interactions.

Malone John H JH Cho Dong-Yeon DY Mattiuzzo Nicolas R NR Artieri Carlo G CG Jiang Lichun L Dale Ryan K RK Smith Harold E HE McDaniel Jennifer J Munro Sarah S Salit Marc M Andrews Justen J Przytycka Teresa M TM Oliver Brian B

Genome biology 20120424 4

<h4>Background</h4>Gene dosage change is a mild perturbation that is a valuable tool for pathway reconstruction in Drosophila. While it is often assumed that reducing gene dose by half leads to two-fold less expression, there is partial autosomal dosage compensation in Drosophila, which may be mediated by feedback or buffering in expression networks.<h4>Results</h4>We profiled expression in engineered flies where gene dose was reduced from two to one. While expression of most one-dose genes was ...[more]

PMID: 22531030

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