Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Effect of floT and floA mutations on the global transcriptome of Bacillus subtilis NCIB3610

ABSTRACT: To compare the altered transcript levels in the floT, floA single and floTfloA double mutants of Bacillus subtilis, cells were grown in MSgg medium until the late exponential phase and their transcriptome was compared to the wild type, NCIB3610 strain. Bacillus subtilis NCIB3610 wild type and its floT, floA, and floTfloA mutants strain were grown in MSgg medium and cells were harvested at late exponential (OD600 ~1.0-1,2) phase. 3 biological replicates were used, resulting 3 slides for each mutant strain. Dye swaps are included in both experiments.

ORGANISM(S): Bacillus subtilis

SUBMITTER: Akos Kovacs

PROVIDER: E-GEOD-47918 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Spatio-temporal remodeling of functional membrane microdomains organizes the signaling networks of a bacterium.

Schneider Johannes J Klein Teresa T Mielich-Süss Benjamin B Koch Gudrun G Franke Christian C Kuipers Oscar P OP Kovács Ákos T ÁT Sauer Markus M Lopez Daniel D

PLoS genetics 20150424 4

Lipid rafts are membrane microdomains specialized in the regulation of numerous cellular processes related to membrane organization, as diverse as signal transduction, protein sorting, membrane trafficking or pathogen invasion. It has been proposed that this functional diversity would require a heterogeneous population of raft domains with varying compositions. However, a mechanism for such diversification is not known. We recently discovered that bacterial membranes organize their signal transd ...[more]

PMID: 25909364

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Project description:Background. Bacillus subtilis is a favorable host for the production of industrially relevant proteins because of its capacity of secreting proteins into the medium to high levels, its GRAS (Generally Recognized As Safe) status, its genetic accessibility and its capacity to grow in large fermentations. However, production of heterologous proteins still faces limitations. Results. This study aimed at the identification of bottlenecks in secretory protein production by analyzing the response of B. subtilis at the transcriptome level to overproduction of eight secretory proteins of endogenous and heterologous origin and with different subcellular or extracellular destination: secreted proteins (NprE and XynA of B. subtilis, Usp45 of Lactococcus lactis, TEM-1beta -lactamase of Escherichia coli), membrane proteins (LmrA of L. lactis and XylP of Lactobacillus pentosus) and lipoproteins (MntA and YcdH of B. subtilis). Responses specific for proteins with a common localization as well as more general stress responses were observed. The latter include upregulation of genes encoding intracellular stress proteins (groES/EL, CtsR regulated genes). Specific responses include upregulation of the liaIHGFSR operon under Usp45 and TEM-1 beta-lactamase overproduction; cssRS, htrA and htrB under all secreted proteins overproduction; sigW and SigW-regulated genes mainly under membrane proteins overproduction; and ykrL (encoding an HtpX homologue) specifically under membrane proteins overproduction. Conclusions. The results give better insights into B. subtilis responses to protein overproduction stress and provide potential targets for genetic engineering in order to further improve B. subtilis as a protein production host. Samples for transcriptome analyses were induced at the exponential-growth phase (OD600 = 0.7) with 0.1% subtilin (subtilin containing supernatant of subtilin producing B. subtilis strain ATCC 6633). Cells were harvested 30 min after induction. Three or four independent cultures of each strain (target strains and controls) were used, and cells were sampled for microarray experiment.

Dataset Information

Effect of floT and floA mutations on the global transcriptome of Bacillus subtilis NCIB3610

Publications

Spatio-temporal remodeling of functional membrane microdomains organizes the signaling networks of a bacterium.

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