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Leucegene

ABSTRACT: This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

ORGANISM(S): Homo sapiens

SUBMITTER: Guy Sauvageau

PROVIDER: E-GEOD-48173 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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RNA-Seq reveals spliceosome and proteasome genes as most consistent transcripts in human cancer cells.

Macrae Tara T Sargeant Tobias T Lemieux Sébastien S Hébert Josée J Deneault Eric E Sauvageau Guy G

PloS one 20130917 9

Accurate quantification of gene expression by qRT-PCR relies on normalization against a consistently expressed control gene. However, control genes in common use often vary greatly between samples, especially in cancer. The advent of Next Generation Sequencing technology offers the possibility to better select control genes with the least cell to cell variability in steady state transcript levels. Here we analyze the transcriptomes of 55 leukemia samples to identify the most consistent genes. Th ...[more]

PMID: 24069164

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