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Transcriptome of Primitive Human Hematopoietic Cells: A New Resource to Find hHSC-Specific Genes

ABSTRACT: We analysed the transcriptome of different HSC-enriched subpopulations of cells sorted from human umbilical cord blood and isolated from several individuals with different genetic backgrounds. We aim at identifying new cell surface markers associated with human HSC and downstream mature hematopoietic cell activity. RNA-seq of CD34+CD45RA- cord blood cells from 17 non-pooled individuals.

ORGANISM(S): Homo sapiens

SUBMITTER: Guy Sauvageau

PROVIDER: E-GEOD-48846 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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RNA-Seq reveals spliceosome and proteasome genes as most consistent transcripts in human cancer cells.

Macrae Tara T Sargeant Tobias T Lemieux Sébastien S Hébert Josée J Deneault Eric E Sauvageau Guy G

PloS one 20130917 9

Accurate quantification of gene expression by qRT-PCR relies on normalization against a consistently expressed control gene. However, control genes in common use often vary greatly between samples, especially in cancer. The advent of Next Generation Sequencing technology offers the possibility to better select control genes with the least cell to cell variability in steady state transcript levels. Here we analyze the transcriptomes of 55 leukemia samples to identify the most consistent genes. Th ...[more]

PMID: 24069164

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Transcriptome of Primitive Human Hematopoietic Cells: A New Resource to Find hHSC-Specific Genes

Project description:We analysed the transcriptome of different HSC-enriched subpopulations of cells sorted from human umbilical cord blood and isolated from several individuals with different genetic backgrounds. We aim at identifying new cell surface markers associated with human HSC and downstream mature hematopoietic cell activity.

2013-11-06 | GSE48846 | GEO

Expression data from human CD34+ HPC subpopulations transduced with nuclear-trapped AF1q/MLLT11 (A2M)

Project description:We used microarrays to examine the impact of AF1q/MLLT11 on the gene expression profile of CD34+CD45RA-Lin- and CD34+CD45RA+Lin- HPCs isolated from umbilical cord blood CD34+CD45RA-Lin- and CD34+CD45RA+Lin- HPCs correspond respectively to early multipotent and lympho-granulo-macrophagic precursors. A2M is a nuclear mutant-derivative of AF1q/MLLT11 CD34+CD45RA-Lin- and CD34+CD45RA+Lin- HPCs were FACS-sorted, exposed to A2M or control vectors, sorted based on GFP expression, and subjected to global gene expression analysis 72 hrs later.

2011-05-26 | E-GEOD-29523 | biostudies-arrayexpress

Expression data from human CD34+ HPC subpopulations isolated from umbilical cord blood (Haddad et al. Blood 104:3918, 2004)

Project description:We used microarrays to analyze the gene expression profile of CD34+CD45RA+CD7+, CD34+CD45RA+CD10+CD19- and CD34+CD45+CD7-CD10-CD19- HPCs isolated from umbilical cord blood CD34+CD45RA+CD7+(CD10-) and CD34+CD45RA+CD10+(CD7-CD19-) HPCs correspond respectively to prothymocytes and early pre-proB precursors. CD34+CD45RA+CD7-CD10-CD19- HPCs correspond to lympho-granulo-macrophagic precursors The corresponding populations were sorted from total CD34+ HPCs isolated from 2 or 3 individual donors

2011-05-25 | E-GEOD-29522 | biostudies-arrayexpress

RNA Sequencing of LT-HSCs and ST-HSCs from healthy adult human Bone Marrow

Project description:The transcriptome of LT-HSC (CD34+CD38-CD45RA+CD90+CD49f+) and ST-HSC (CD34+CD38-CD45RA+CD90-CD49f-) from healthy adult human Bone Marrow Cells were assessed by RNA-seq.

2022-04-06 | E-MTAB-9892 | biostudies-arrayexpress

Expression data from Notch-activated CD34+CD45RA-Lin- hematopoietic progenitor cells (HPCs) transduced with nuclear-trapped AF1q/MLLT11 (A2M)

Project description:We used microarrays to examine the impact of AF1q/MLLT11 on the gene expression profile of Notch-activated CD34+CD45RA-Lin- HPCs isolated from umbilical cord blood CD34+CD45RA-Lin- HPCs correspond to early multipotent progenitors. A2M is a nuclear mutant derivative of AF1q/MLLT11 CD34+CD45RA-Lin- HPCs were FACS-sorted, exposed to A2M or control vectors, sorted again based on GFP expression, and cultured for 72 hrs with graded doses of plastic-immobilized Notch ligand Delta1ext-IgG

2011-05-26 | E-GEOD-29524 | biostudies-arrayexpress

Comparing gene expression in stem/progenitor cells from patients with CML in chronic, accelerated and blastic phase with normal volunteers

Project description:A comparison of global gene expression between rigorously defined stem and progenitor cells from patients with chronic myeloid leukaemia (CML) in chronic (CP), accelerated (AP) and blastic (BC) phase and similar populations isolated from normal volunteers. Cryopreserved CD34+ enriched cell populations obtained from patients with CML in CP, AP or BC at diagnosis prior to treatment -- or from normal volunteers -- were thawed and flow sorted into rigorously defined sub-populations (HSC, MPP, CMP, GMP and MEP -- using surface phenotype as described below). Total RNA was obtained and global gene expression measured following hybridisation to Affymetrix HuGene-1_0-st-v1 gene-chips. In total, 3 normal patient samples were compared with 6 CP, 4 AP and 2 BC samples. HSC, CMP, GMP and MEP populations were obtained from all specimens, MPP populations were obtained from normal, AP and BC specimens but not CP specimens.

2013-09-06 | E-GEOD-47927 | biostudies-arrayexpress

Functional exhaustion of fetal Th1 lymphocytes following congenital CMV infection - Huygens A. Marchant A.

Project description:Comparison of the transcriptome of naive (CD45RA+ CD27+) and differentiated (CD27-CD28-) CD4+ lymphocytes from newborns with congenital human cytomegalovirus infection (cord blood samples) and pregnant women diagnosed with primary CMV infection. Naive cells from HCMV uninfected newborns were used as a control.

2015-01-18 | E-MEXP-3473 | biostudies-arrayexpress

Isolation of single human hematopoietic stem cells capable of long-term multilineage engraftment

Project description:Gene expression profiling was used to investigate the relationship between human cord blood hematopoietic stem cells (HSC) and multipotent progenitors described in the study. Genes more highly expressed in HSC may be associated with stem cell function and self-renewal. Total RNA was obtained from flow-sorted populations based on the cell surface expression of CD34, CD38, CD45RA, Thy1, and CD49f.

2011-07-31 | E-GEOD-29105 | biostudies-arrayexpress

Single Cell RNA Sequencing of human bone marrow LT-HSCs.

Project description:10x single cell sequencing of LT-HSCs (CD34+CD38-CD90+CD45RA-CD49f+) from human bone marrow.

2022-04-06 | E-MTAB-9922 | biostudies-arrayexpress

Transcription profiling of human umbilical cord blood and adult bone marrow CD34+CD33-CD38-Rho(lo)c-kit+ cells to identify candidate regulators of HSC self-renewal versus differentiation fate decisions

Project description:The global gene expression profiles of human umbilical cord blood and adult bone marrow CD34+CD33-CD38-Rho(lo)c-kit+ cells, enriched for hematopoietic stem/progenitor cells (HSC) with CD34+CD33-CD38-Rho(hi) cells, enriched in committed hematopoietic progenitor cells (HPC), were compared to identify candidate regulators of HSC self-renewal versus differentiation fate decisions.

2007-10-30 | E-GEOD-2666 | biostudies-arrayexpress

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